茸菖胶囊对戊四唑点燃模型大鼠海马BDNF及其受体TrkB表达的影响

Effects of Rongchang capsules on expression of brain-derived neurotrophic factor and its TrkB in hippocampus after pentylenetetrazol-induced epileptic seizure in rats

[目的]阐明脑源性神经营养因子(BDNF)及其功能受体酷氨酸激酶B(TrkB)在癫痫后认知障碍中的作用以及茸菖胶囊对海马脑源性神经营养因子及其受体TrkB的远期影响。[方法]以戊四唑点燃大鼠为模型,随机分为中药组、西药组、模型组和正常组,观察致痫大鼠的惊厥发作次数、级别、潜伏期,采用RT-PCR检测BDNF和TrkB基因表达水平。[结果]各治疗组大鼠与治疗前相比,均可减少大鼠的发作程度,治疗前造模各组的发作次数无统计学差异(P>0.05),茸菖胶囊治疗后,治疗大鼠的发作评分明显下降,与模型组相比,各治疗组的发作评分较低(P<0.05),茸菖胶囊组与丙戊酸钠组的评分无统计学差异(P>0.05)。各组海马组织BDNF mRNA表达显示,模型组海马组织BDNFmRNA表达较正常组、中药组和VPA组明显增多,中药组表达较其他各组增多均有统计学差异(P<0.01)。海马组织TrkB mRNA表达与BDNF mRNA表达趋势相同。[结论]茸菖胶囊可以有效控制癫痫大鼠的发作次数及级别,其作用机制与调控海马组织中BDNF、TrkB mRNA的表达有关。

[Objective] To explore the effect of Rongchang capsules （RCC） on the expression of brain-derived neurotrophic factor （BDNF） and its TrkB in hippocampus after pentylenetetrazol-induced epileptic seizure in rats. [Methods] The 40 rats were randomly divided into normal group, model group, valproic acid sodium （VPA） group and RCC group. The model of epileptic rats was kindled by PTZ. We ob- served the frequence of epileptic seizures, levels, and the incubation period. Using RT-PCR method the expression level of BDNF and its TrkB in the hippocampus were detected.[Results] Compared with before treatment, the attack degree in treatment rats was reduced. Be- fore modeling the number of attacks of each moled groups had no significant difference （P〉O.05）. After RCC treatment, the attack score was significantly decreased compared with model group. The onset score of each treatment groups was lower （P〈0.05）. There was no sig- nificant difference between RCC group and VPA group（P〉O.05）. The results of expression of BDNF in hippocampus tissue showed that the expression of BDNF mRNA in hippocampus tissue was significantly higher than the normal group, the Chinese medicine group and VPA group. The expression in Chnese medicine was increased significantly more than other groups （P〈0.01）. The trend of the expression of TrkB mRNA was the same as the expression of BDNF. [Conclusion] RCC can effectively control the attack frequency and level of the epilepsy and improve the ability of learning and memory of epilepsy in rats. The mechanism may be associated with the regulation of BD- NF and TrkB expressions.