固定多黏菌素B的压电生物传感器检测内毒素

Detection of endotoxin using polymyxin B-coated piezoelectric biosensor

目的本研究拟探讨快速、定量和灵敏的内毒素(ET)检测方法。方法通过硅烷化的晶体表面将多黏菌素B(PMB)固定到ET切割10MHz压电石英晶体表面,研制成一种新型的用于检测血浆中ET浓度的压电生物传感器。结果该传感器检测中,ET在0.5～20.0μg/mL浓度范围内有良好的线性关系。结论该研究探讨了PMB浓度、溶液的离子强度和pH值以及反应时间等对ET吸附性能的影响,检测结果表明该传感器可用于临床和环境检测。

Objective To discuss the rapid,quantitative and sensitive detective method on endotoxin（ET）. Methods Polymyxin B, which hinded with ET, was immobilized onto the silanized modified 10 MHz AT-cut quartz crystal resonator. The biosensor was developed for detection of ET in plasma. Results The linear correlation could be obtained from 0.5 to 20 btg/mL of ET for this pie- zoelectric biosensor. Conclusion The effect of concentration of polymyxin B,ionic strength and pH of solution and reaction time on absorption ability,on ET adsorbability was studied. The results suggest the biosensor is potentially useful both in clinical and environmental detection.