MG53对重度烫伤小鼠肾脏保护作用的研究

Protection of MG53 on kidney in mice with severe burn injury*

目的探讨Mitsugumin 53(MG53)对重度烫伤小鼠内脏器官的保护作用。方法通过向30%Ⅲ度烫伤小鼠的尾静脉注射外源性重组的人MG53(rhMG53)蛋白,观察小鼠病死率和各脏器组织改变,并用免疫组织化学法检测外源性rhMG53蛋白的分布。结果与对照组比较,注射rhMG53的小鼠病死率较低,差异无统计学意义(P>0.05)。组织病理学观察,实验组静脉注射MG53后肾脏损伤情况较轻,肾小管损伤病理评分较低,与对照组比较差异有统计学意义(P<0.05)。免疫组织化学发现外源性rhMG53分布于部分肾小管上皮细胞胞质内以及部分肺小静脉内皮细胞胞质内。其他脏器组织学未见没见有明显的rh-MG53阳性染色。结论 MG53对重度烫伤小鼠的肾脏有一定的保护作用。

Objective To understand the possible roles of Mitsugumin53 （MG53） in protection of internal organs after severe burn injury. Methods A burn model of 30% of total body surface area （TI3SA） was set up as reported. Recombinant human MG53 （rhMG53） protein was intravenously injected via tail vein. The mortality, histological alteration and immunohistochemical staining were observed and recorded. Results The mortality in rhMG53 protein injection group was lower than that in control group. How- ever,there was no statistically significant difference （P）-0.05）. rhMG53 administration could apparently alleviate histological alter- ation of renal tubular epithelial cells after burn injury. The renal tubular injury scores of MG53 treated group were lower than that of control group （P〈0.05）. Immunohistochemical staining found that exogenous rhMG53 was located in renal tubular epithelial cells and pulmonary intravenous endothelial cells. Conclusion MG53 could protect kidney from severe burn injury.