少突胶质瘤染色体1p/19q联合缺失与Ki-67表达的关系

Correlation research between loss of chromosome 1p/19q and protein expression of Ki-67 of oligodendrocytes glioma

目的观察少突胶质细胞瘤1号染色体短臂(1p)和19号染色体长臂(19q)联合杂合性缺失(LOH)与Ki-67蛋白表达的相关性,探索预测少突胶质瘤化疗敏感性的分子标记物。方法少突胶质瘤肿瘤组织标本31例作为实验组,少突胶质瘤瘤旁正常脑组织标本13例作为对照组,免疫组化方法检测两组Ki-67蛋白的表达,荧光原位杂交技术检测1p/19q的缺失情况。结果对照组有1例Ki-67表达呈现阳性(7.7%),实验组有14例Ki-67表达呈现阳性(45.0%),两组阳性率差异有统计学意义(P<0.05);对照组无1p/19q缺失,实验组有13例(41.9%)1p/19q联合缺失,其中1p单独缺失2例(6.4%),19q单独缺失1例(3.2%)。Ki-67蛋白表达与1p/19q联合缺失显著相关(r=0.403,P<0.05)。结论 Ki-67蛋白表达是预测少突胶质瘤化疗敏感性的潜在分子标记物。

Objective To observe oligodendrocytes gliomas chromosome 1p/19q deletion and Ki-67 protein expression,explore new molecular markers forecasting chemosensitivity of oligodendrocytes glioma. Methods 31 oligodendrocytes glioma specimens as the experimental group, 13 cases adjacent normal brain tissue of oligodend＇rocytes glioma as a control group, the Ki-67 protein detec- ted by immunohistochemistry and lp/19q detection detected by fluorescence in situ hybridization. Results Compared with control group,the experimental group had 14 cases of Ki-67 expression with positive rate 45%;lp/19q deletions was not detected in the control group, 1 p/19q loss for 13 cases（41.9 %） in the experimental group of 31 cases of oligodendroglioma glioma specimens, lp a- lone missing for 2 cases（6.4%）,19q alone missing for 1 case（3.2%）. Ki-67 protein expression and the lp/19q loss was significant correlation（P〈0.05）. Conclusion Ki-67 protein expression in the gliomas may be as the potential molecular markers of chemosen- sitivity oligodendrocytes gliomas.