促凋亡基因程序化死亡因子5在非小细胞肺癌中的表达及意义

Expressions and significances of promoting apoptosis gene programmed cell death 5 in non-small cell lung cancer

目的:研究程序化死亡因子5(programmed cell death 5,PDCD5)基因在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达,探讨其在NSCLC中发生、发展中的作用。方法:随机选取2011年7月至12月南昌大学第一附属医院胸外科手术切除并经病理证实为NSCLC的56例患者的癌组织及癌旁组织标本,采用免疫组化及real-time PCR方法研究在NSCLC及癌旁组织中PDCD5蛋白和mRNA的表达。结果:免疫组化结果显示,癌旁肺组织中PDCD5阳性表达率高于NSCLC组织(χ2=16.406,P=0.000)。与抽烟情况、肿瘤分化程度、肿瘤TNM分期及有无淋巴结转移密切相关,差异均有统计学意义,并随抽烟、分化程度的下降、TNM分期的升高及淋巴结转移,其表达随之下降,同时染色强度也减弱。Real-time PCR结果,PDCD5 mRNA相对表达量在肺癌组织为0.397±0.266,癌旁组织为0.807±0.124,差异有统计学意义(t=10.795,P=0.000)。结论:在NSCLC中PDCD5蛋白及mRNA表达均下调,在肺癌的发生、发展中可能起抑制作用。

Objective：To investigate expressions of programmed cell death 5 （PDCD5） in non-small cell lung cancer（NSCLC） and to explore its role in NSCLC occurrence and development. Methods： Cancer tissue and adjacent cancer tissue samples of 56 patients with NSCLC who received thoracic surgery and pathology confirmation from July 2011 to December in the first affiliated hospital of Nan- chang University were collected. Expressions of PDCD5 protein and mRNA were evaluated by immunohistochemistry and real-time PCR in NSCLC and adjacent cancer tissues. Results：Immunohistochemical results showed that PDCD5 positive expression rate was significantly higher in adjacent cancer tissues than in NSCLC tissues（x2=16.406,P=0.000）. PDCD5 positive expression rate was closely related with smoking,tumor differentiation,tumor TNM stage and lymph node metastasis,with statistically significant differ- ences. PDCD5 positive expression rate was decreased with the decline of smoking and differentiation degree and the elevation of TNM staging and lymph node metastasis;meanwhile, intensity of staining was also weaken. Real-time PCR results showed that PDCD5 mRNA relative expression was 0.397 ± 0.266 in NSCLC tissues and 0.807 ± 0.126 in adjacent tissues with statistically significant dif- ferences （t=10.795,P=0.000）. Conclusions：Expressions PDCD5 protein and mRNA are down-regulated,which may play an inhibitory role in NSCLC occurrence and development.