核转录因子-κB和缺氧诱导因子-1α在前列腺癌中的表达及意义

Expressions and clinical significances of nuclear factor-κB and hypoxia inducible factor-1α in prostate cancer

目的:研究核转录因子-κB(nuclear factor kappa B,NF-κB)和缺氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)在前列腺癌中的表达及其临床意义。方法:采用逆转录聚合酶链反应(reverse transcriptase-polymerase chain reaction,RT-PCR)和免疫组织化学光谱纯试剂(spectrum pure,SP)法检测62例前列腺癌组织及17例良性前列腺增生组织中NF-κB和HIF-1α的mRNA和蛋白的表达。结果:(1)RT-PCR结果显示NF-κB和HIF-1α的mRNA在前列腺癌组中的相对表达量明显高于前列腺增生(P<0.05)。免疫组织化学结果显示NF-κB和HIF-1α蛋白在前列腺癌组中的阳性表达率分别为62.90%和66.13%,明显高于前列腺增生组11.77%和5.88%(P<0.05)。(2)NF-κB和HIF-1α的阳性表达均与前列腺癌的Gleason分级、临床分期有关(P<0.05),而与年龄无关(P>0.05)。(3)NF-κB和HIF-1α的表达呈正相关(rs=0.368,P<0.05)。结论:NF-κB和HIF-1α可能与前列腺癌的发生、发展密切相关,并可能在前列腺癌的浸润和转移中起重要作用。

Objective：To study expressions and clinical significances of nuclear factor-κB（NF-κB） and hypoxia inducible factor-1α（HIF-1α） in prostate cancer. Methods：RT-PCR and immunohistochemistry SP method were used to detect NF-KB and HIF-1α mRNA and protein expressions in 62 cases of prostate cancer tissues and 17 cases of prostatic hyperplasia tissues. RestdtE： （ 1 ）RT-PCR results showed that NF-κB and HIF-1α mRNA expression levels were relatively significantly higher in prostate cancer group than in prostatic hyperplasia group, with statistical differences（P〈0.05）. Immunohistochemical results showed that positive expression rates of NF-κB and HIF-1α proteins in prostate cancer group were 62.90% and 66.13%, 8.33% and 5.88%, significantly higher than those in prostatic hyperplasia group with statistically significant differences（P〈0.05）. （2）Expressions of NF-κB and HIF-1α were correlated with Gleason grade and clinical stages of prostate cancer（P〈0.05）, but not with age （P〉0.05）. （3）Expressions of NF-κB and HIF-1α were positively correlated（rs=0.368 ,P〈0.05）. Conclusions ：Overexpression of NF-KB and HIF-1α may be involved in the pathogene- sis and progression of prostate cancer.Moreover, NF-κB and HIF-1α may be correlated with the invasion and metastasis of prostate