siRNA沉默RIP1对膀胱癌细胞株T24恶性生物学行为的影响

Impact on malignant behaviour of bladder cancer cell line T24 after RIP1 was silenced by siRNA

目的利用siRNA转染干扰沉默膀胱癌细胞株T24的受体相互作用蛋白1(RIP1)基因,并观察沉默前后T24恶性生物学行为的变化。方法使用Lipofectamine誖2000和RIP1siRNA与膀胱癌细胞株T24共孵育48 h,RT-PCR和Western-blot检测孵育前后RIP1mRNA和蛋白质的表达变化以观察沉默效率,并观察沉默前后T24增殖能力、克隆形成能力、迁移和侵袭能力的变化。结果与siRNA孵育48 h后,RIP1的沉默效率可以达到85%,RIP1沉默后T24的增殖能力、克隆形成能力、迁移和侵袭能力下降。结论沉默RIP1基因可以降低T24的恶性程度,提示RIP1在膀胱癌T24中起癌基因的作用。

Objective To study the impact on malignant behaviour of bladder cancer cell line T24 after RIP1 was silenced by siRNA.Methods After incubation T24 cell with Lipofectamine  2000 and siRNA,we used RT-PCR and Western blot to compare expression of RIP1.We also compared the ability of proliferation,colony formation,mobility and invasion of T24 after RIP1 silenced by siRNA.Results After incubation with siRNA for 48 hours,expression of RIP1 was decreased to 15% and the ability of proliferation,colony formation,mobility and invasion of T24 was decreased.Conclusion RIP1 is an oncogene in T24.