肝癌组织中Vasohibin1和HIF-1α表达及临床意义

The clinical significance and the expression of Vasohibin1 and HIF-1α in hepatocellular carcinoma

目的研究肝癌组织中血管生成抑制蛋白(Vasohibin1)和缺氧诱导因子-1α(HIF-1α)的表达及两者的相关性,并探讨其临床意义。方法随机收集64例原发性肝细胞肝癌手术患者的癌组织,另选其中的26例癌旁组织作为对照组,应用免疫组织化学法检测Vasohibin1与HIF-1α在肝癌组织与癌旁组织中的表达特征并进行评分,进一步分析二者间的相关性及与肝癌患者临床病理特征间的关系。结果肝细胞肝癌组织中Vasohibin1和HIF-1α表达升高,与癌旁组织比较差异有统计学意义(χ2=20.19,P=0.00;χ2=5.147,P=0.023)。结合肝癌患者临床病理特征的统计分析表明,Vasohibin1的高表达与肝癌各临床病理参数在统计学上无明显相关性(P>0.05),HIF-1α的高表达与有无肿瘤转移有关(χ2=4.882,P=0.027),并且肝癌组织中二者的表达在统计学上有相关性(r=0.481,P=0.00)。结论 Vasohibin1和HIF-1α在肝癌组织高表达,提示二者在肝癌发生发展过程中发挥作用;二者的表达相关性提示它们之间可能以VEGF作为纽带或者存在相互作用。

Objective To investigate the expression and clinical significance of Vasohibinl and HIF-1α in hepato- cellu1αr carcinoma （HCC） , and study the correlationof Vasohibinl and HIF-1α. Methods Immunohistochemistry was used to detect the expression of Vasohibinl and HIF-1α in 64 HCC tissues, 26 adjacent non-tumor tissues. Furthers, statistic analysis was conducted to value their re1αtion with the cliniealpathological parameters and the correlationbetween themselves. Results Vasohibinl and HIF-1α were over-expressed in HCC compared with the adjacent non-tumor tissues （X^2 = 20.19, P = 0. 00 ; X^2 = 5. 147, P = 0. 023）. Further, analysis of their re1αtionship with cinicalpathological parameters suggested that the expression of Vasohibinl wasn＇ t re1αted to the clinical pathological parameters （P 〉 0.05 ） and HIF-1α expression was re1αted to tumor metastasis （X^2 = 4. 882, P = 0. 027）. The expression of Vasohibinl and HIF-1α in HCC were statistically significantly corre1αted （r = 0. 481 ,P = 0.00）. Conclusion Vasohibinl and HIF-1α protein in HCC were highly expressed and they p1αy key roles in the development of HCC. Statistics showed that there was a correlationbetween Vasohibinl and HIF-1α.