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柚皮苷纳米纤维膜载药量影响成骨细胞的增殖和分化 被引量:6

Drug loading of naringin nanofibrous membrane influences osteoblast proliferation and differentiation
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摘要 背景:多数研究认为,单纯的引导性骨再生膜只是起到了机械性阻挡和隔离作用,不具备主动诱导作用。大量研究显示柚皮苷可促进多种成骨样细胞的增殖和分化。目的:制备载有柚皮苷的引导性骨再生膜,并研究一定电纺参数下的最佳载药剂量。方法:以聚己内酯与聚乙二醇-b-聚己内酯为材料,通过静电纺丝技术制备含0(对照),0.1,1,10,100g/L柚皮苷的纳米纤维膜,扫描电镜观察膜的表面形态。将MC3T3-E1成骨细胞接种于含不同质量浓度柚皮苷的纳米纤维膜上,以扫描电镜观察细胞/膜复合体的表面形态,以噻唑蓝法检测细胞的增殖能力,以碱性磷酸酶活性检测评估细胞的分化能力。结果与结论:当柚皮苷质量浓度为0.1,1,10g/L时,纳米纤维表明光滑,直径均一,呈纵横交错的三维网状结构。当柚皮苷质量浓度100g/L时,纳米纤维膜表面有较多膨大的珠状结构,直径均一性较差。与对照组比较,含10g/L柚皮苷的纳米纤维膜能显著促进成骨细胞的增殖(P<0.05);含1,10g/L柚皮苷的纳米纤维膜均能显著促进成骨细胞的碱性磷酸酶活性(P<0.05),并且以含10g/L柚皮苷的纳米纤维膜组作用最明显。表明载柚皮苷的引导性骨再生纳米纤维膜能促进成骨细胞的增殖和分化,10g/L为柚皮苷的最佳载药剂量。 BACKGROUND: Guided bone regeneration membrane just plays a mechanical barrier and isolation effect, but cannot exert an active induction role. Numerous studies have shown that naringin can promote theproliferation and differentiation of a variety of osteoblast-like cells. OBJECTIVE: To prepare the guided bone regeneration membranes carrying naringin and to design the optimal drug loading of the membrane. METHODS: Nanofibrous membranes containing different contents of naringin (0, 0.1, 1, 10, 100 g/L) were prepared by electrospinning. The morphologies of the membranes were observed by scanning electron microscope. MC3T3-E1 osteoblasts were seeded on the membranes. The morphologies of the cell-membrane composites were observed by scanning electron microscope. The cell proliferation was tested by methyl thiazolyl tetrazolium assay and the cell differentiation was evaluated by alkaline phosphatase activity. RESULTS AND CONCLUSION: Smooth and uniform nanofibers were obtained by electrospinning with drug loading of 0.1, 1, 10 g/L naringin. There were no significant differences among the diameters of these three kinds of nanofibers. Many beads were observed in the nanofibrous membrane containing 100 g/L naringin. The results of methyl thiazolyl tetrazolium assay and alkaline phosphatase activity showed, as compared with the control group (containing 0 g/L naringin), the nanofibrous membrane containing 10 g/L naringin significantly promoted cell proliferation and differentiation (P 〈 0.05), while that containing 1 and 10 g/L naringin significantly increased alkaline phosphatase activity in osteoblasts (P 〈 0.05). The electrospinning naringin nanofibrous membrane with the optimal drug loading of 10 g/L promoted osteoblast proliferation and differentiation most significantly. Therefore, this new type of guided bone regeneration membranes will have a wonderful prospect in repair of bone defect under the state of osteoporosis.
出处 《中国组织工程研究》 CAS CSCD 2013年第25期4577-4584,共8页 Chinese Journal of Tissue Engineering Research
基金 重庆市科委(部省级面上项目)课题:控释柚皮苷纳米纤维膜修复骨质疏松状态下的骨临界缺损的研究(CSTC 2010BB5102) 重庆市医科大学校级课题(国家自然科学基金预研项目):柚皮苷对骨质疏松状态下颅颌面骨缺损修复的机理研究(NSFYY201045) 重庆医科大学附属口腔医院院级课题:控释中药的诱导骨再生膜的研制~~
关键词 生物材料 组织工程骨材料 纳米材料 电纺 柚皮苷 纳米纤维膜 载药 成骨细胞 增殖 分化 省级基金 K biomaterials tissue-engineered bone materials nanomaterials electrospinning naringin nanofibrous membranes drug-loading osteoblasts proliferation differentiation provincial grants-supportedpaper
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