水飞蓟宾减轻庆大霉素肾毒性作用的研究

Silybin attenuated the nephrotoxic effects of gentamicin research

目的:观察水飞蓟宾减轻庆大霉素引起的肾脏损伤的作用。方法:采用雄性豚鼠50只,随机分为空白对照、庆大霉素和水飞蓟宾低剂量组,水飞蓟宾高剂量组。连续给药10天后,ILISA检测肾组织中MDA(脂质过氧化物),SOD(超氧化物歧化酶),GSH-Px(谷胱甘肽过氧化物酶)和CAT(过氧化氢酶)含量。用透射电镜检测肾脏近曲小管的超微结构。结果:水飞蓟宾(50 mg/kg,100mg/kg)能够显著诱导肾脏细胞内抗氧化酶GSH-Px、CAT和SOD的生成,同时降低MDA的含量。电镜结果显示水飞蓟宾高剂量(100 mg/kg)组明显改善肾小管超微结构。结论:水飞蓟宾可诱导肾脏细胞内抗氧化酶的生成,减少庆大霉素产生的活性氧,进而减轻庆大霉素引起的肾脏毒性。

To observe silybin effect on gemamicin-induced renal injury in Guinea pigs. Methods： 50 male guinea pigs were randomly di- vided into control, gentamicin, silybin low-dose group （50 mg/kg, 100mg/kg）and silybin high-dose groups （100 mg/kg）. At day 10 after administration, ILISA was used to detect the MDA, SOD, GSH-PX and CAT levels in renal tissue of the Guinea pigs. Transmission electron microscopy was used to observe the renal proximal tubule ultrastructure changes in the Guinea pigs. Results： Silybin （50mg/Kg） induced antioxidant enzymes of GSH-PX, CAT and SOD generation in the renal cells of the Guinea pigs, while the content of MDA was reduced. Elec- tron microscope showed that silybin high dose （100mg/kg） was significantly improved tubular ultra structure. Dose range ： 50 - 100mg/kg. Conclusion： Silybin can induce the formation of antioxidant enzymes in kidney cells, reduce gentamicin-generated reactive oxygen species, and thus reduce the gentamicin-induced renal toxicity.