瓜蒌-薤白对慢性心肌缺血的保护作用

Protective effects of Trichosanthes kirilowii and Allium macrostemon on chronic myocardial ischemia

目的:通过建立异丙肾上腺素(ISO)慢性心肌缺血(chronic myocardial ischemia)大鼠模型,研究瓜蒌、薤白、瓜蒌-薤白对慢性心肌缺血的保护作用。方法:采用腹腔注射异丙肾上腺素制造大鼠心肌缺血模型,记录造模前、造模后、给药后的心电图,观察心电图ST段变化。给药两周后,大鼠断头取血,测血清中乳酸脱氢酶(LDH)、肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)水平;对动物的心脏组织进行HE染色,电镜切片观察组织病理变化。结果:瓜蒌-薤白煎液18.75g/kg～37.5g/kg能够有效降低心肌缺血引起的心电图ST段抬高,降低血清中CK-MB含量,改善缺血模型造成的心肌纤维紊乱、细胞空泡、细胞器肿胀。结论:瓜蒌-薤白对慢性心肌缺血有保护作用,瓜蒌-薤白各部分指标改善均优于瓜蒌、薤白单用。

Through the establishment of isoproterenol （ISO） ischemic chronic myocardial isehemia rat model to investigate the effects of Trichosanthes Kifilowii Maxim （TK）and Allium maerostemon Bge （AM） on chronic myocardial ischemia. Methods： Manufacture the models of chronic myocardial ischemia by intraperitoneal injection of isoproterenel, and recorded the EGG of before modeling, after modeling, after administration , observe changes of electrocardiogram ST segment. After two weeks, breakage of rat blood sampling, measuring levels of lac- tate dehydrogenase （LDH）, creatine kinase （CK）and ereatine kinase-MB（CK-MB）. HE staining of the heart tissue of rats. Observe myocar- dial histopathological changes by electron microscopy. Result： TKAM can effectively reduce ST segment elevation, lower levels of CK-MB, improve the myocardial fibers disorder,vacuolar, organelle swelling which caused by ischemia. Conclusion： TKAM has protective effect on chronic myocardial ischemia. And TKAM are better than TK or AM alone.