摘要
目的:探讨梓醇对高糖诱导SH-SY5Y神经细胞凋亡的保护作用及其机制。方法:将细胞随机分为对照组、凋亡组、梓醇组、梓醇保护组及甘露醇组。凋亡组用含50 mmol/L葡萄糖的培养液培养SH-SY5Y细胞48 h;梓醇保护组的培养液中分别加入1、2、4 mg/ml梓醇和50 mmol/L葡萄糖,培养48 h。通过MTT比色法检测细胞存活率,Hoechst-33258染色观察细胞核形态变化,流式细胞仪检测细胞凋亡率,酶联免疫吸附法检测细胞上清液中8-羟基脱氧鸟苷酸(8-hydoxydeoxyguanosine,8-OHdG)的含量。结果:与对照组相比,凋亡组细胞存活率明显降低;细胞凋亡率明显升高;Hoechst33258染色细胞核出现凋亡小体;上清中8-OHdG含量明显升高。与凋亡组相比,1,2,4 mg/ml梓醇保护组细胞存活率均明显升高;Hoechst33258染色发现细胞核形态明显改善,细胞核固缩减少;梓醇保护组细胞凋亡率及上清液中8-OHdG的分泌量均明显降低。结论:梓醇对高糖诱导的SH-SY5Y细胞凋亡具有保护作用,其机制可能与梓醇的抗氧化作用有关。
Object:To investigate the protective effects of catalpol on high glucose induced apoptosis and its mechanism. Methods: The cells were randomly divided into control group,apoptosis group,catalpol group,catalpol protected group and mannitol group. The apoptosis of SHSY5Y was induced by 50 mmol/L glucose for 48 hours,catalpol protected group be composed of the different final concentration of catalpol (1,2,4 mg/ml) and glucose 50 mmol/L were added together for 48 hours. The cell viability was analyzed by MTT assay; the change of nuclear was assayed by Hoechst 33258; the apoptosis rate was measured by flow cytometry. The level of 8-OHDG was determined by enzymelinked immunosorbent assay. Results: Compared with control group,the apoptosis group's cell viability was decreased; the early apoptosis rate and the content of 8-OHDG were increased; karyopyknosis,split and apoptosis were found by Hoechst33258 dyeing. Compared with apoptosis group group,the cell viability of catalpol ( 1,2,4 mg/ml) protect groups were obviously increased; the early apoptosis rate and the level of 8-OHDG were significantly decreased; the nuclear shape was clearly ameliorated. Conclusion: The results show that Catalpol has protective effect on high glucose induced apoptosis; the mechanism may be related to its antioxidation.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2013年第3期43-46,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
辽宁省教育厅创新团队项目计划(2008T115)