梓醇对高糖诱导SH-SY5Y细胞凋亡的保护作用

Protective effects of catalpol on high glucose induced apoptosis in SH-SY5Y

目的:探讨梓醇对高糖诱导SH-SY5Y神经细胞凋亡的保护作用及其机制。方法:将细胞随机分为对照组、凋亡组、梓醇组、梓醇保护组及甘露醇组。凋亡组用含50 mmol/L葡萄糖的培养液培养SH-SY5Y细胞48 h;梓醇保护组的培养液中分别加入1、2、4 mg/ml梓醇和50 mmol/L葡萄糖,培养48 h。通过MTT比色法检测细胞存活率,Hoechst-33258染色观察细胞核形态变化,流式细胞仪检测细胞凋亡率,酶联免疫吸附法检测细胞上清液中8-羟基脱氧鸟苷酸(8-hydoxydeoxyguanosine,8-OHdG)的含量。结果:与对照组相比,凋亡组细胞存活率明显降低;细胞凋亡率明显升高;Hoechst33258染色细胞核出现凋亡小体;上清中8-OHdG含量明显升高。与凋亡组相比,1,2,4 mg/ml梓醇保护组细胞存活率均明显升高;Hoechst33258染色发现细胞核形态明显改善,细胞核固缩减少;梓醇保护组细胞凋亡率及上清液中8-OHdG的分泌量均明显降低。结论:梓醇对高糖诱导的SH-SY5Y细胞凋亡具有保护作用,其机制可能与梓醇的抗氧化作用有关。

Object：To investigate the protective effects of catalpol on high glucose induced apoptosis and its mechanism. Methods： The cells were randomly divided into control group,apoptosis group,catalpol group,catalpol protected group and mannitol group. The apoptosis of SHSY5Y was induced by 50 mmol/L glucose for 48 hours,catalpol protected group be composed of the different final concentration of catalpol （1,2,4 mg/ml） and glucose 50 mmol/L were added together for 48 hours. The cell viability was analyzed by MTT assay; the change of nuclear was assayed by Hoechst 33258; the apoptosis rate was measured by flow cytometry. The level of 8-OHDG was determined by enzymelinked immunosorbent assay. Results： Compared with control group,the apoptosis group＇s cell viability was decreased; the early apoptosis rate and the content of 8-OHDG were increased; karyopyknosis,split and apoptosis were found by Hoechst33258 dyeing. Compared with apoptosis group group,the cell viability of catalpol （ 1,2,4 mg/ml） protect groups were obviously increased; the early apoptosis rate and the level of 8-OHDG were significantly decreased; the nuclear shape was clearly ameliorated. Conclusion： The results show that Catalpol has protective effect on high glucose induced apoptosis; the mechanism may be related to its antioxidation.