苦参素对大鼠急性酒精性肝损伤的保护作用及其作用机制

Protective effects and mechanisms of matrine on acute alcoholic liver injury in rats

目的:研究苦参素对大鼠急性酒精性肝损伤的保护作用及作用机制。方法:构建大鼠急性酒精性肝损伤模型,测定造模第3日、第5日血清谷丙转氨酶(ALT)和谷草转氨酶(AST);测定第5日肝组织超氧化物歧化酶(SOD)、丙二醛(MDA)、还原型谷胱甘肽(GSH)和谷胱甘肽过氧化物酶(GSH-Px)的水平;测定肝细胞凋亡程度及肝脏沉默信息调节因子1(Sirt1)的mRNA及蛋白表达。结果:大鼠急性酒精性肝损伤后第3日和第5日血清ALT、AST水平升高;肝组织SOD、GSH、GSH-Px降低,MDA升高;细胞凋亡显著;Sirt1的mRNA和蛋白表达降低。苦参素40 mg/kg组和苦参素80 mg/kg组能不同程度减轻肝损伤,其机制可能与苦参素提高了Sirt1的mRNA和蛋白表达相关。结论:苦参素在大鼠急性酒精性肝损伤中发挥保护作用,其机制可能与提高肝脏Sirt1的mRNA和蛋白表达相关。

Objective：To investigate the protective effect of matrine on acute alcoholic liver injury in rats and its mechanism.Methods： Establish the model of acute alcoholic liver injury in rats.The salanine aminotransferase（ALT）,aspartate aminotransferase（AST） in the serum,the hepatic superoxide dismutase（SOD）,malondialdehyde（MDA）,glutathione（GSH）,glutathione peroxidase（GSH-Px）,the hepatic apoptosis,and silent information regulator factor 1（Sirt1） mRNA and protein expression in the liver were measured.Results： we detected the serum ALT and AST were elevated in rats of acute alcoholic liver injury,together with hepatic SOD,GSH and GSH-PX decreased;MDA and apoptotic positive index increased.Moreover,hepatic Sirt1 mRNA and protein expressions were suppressed.Such damages were alleviated in matrine pretreatment group（40 mg/kg,80 mg/kg）,the potential mechanism may be related to Sirt1 mRNA and protein expressions were elevated by matrine pretreatment.Conclusion： Matrine has significant protective effects on acute alcoholic liver injury in rats.The mechanism of its protective effects may relate to its induced property of Sirt1 mRNA and protein.