喹啉降解菌BW004的分离、鉴定及降解特性

Isolation,Identification,and Metabolic Characteristics of a Quinoline Degrading Bacterium

从武汉钢铁（集团）公司焦化废水处理厂的好氧活性污泥中分离出一株细菌,可利用喹啉作为唯一的碳、氮和能源进行生长。经16S rRNA测序鉴定为假单胞菌属,命名为Pseudomonas sp.BW004。利用响应曲面法确定其最佳降解条件,为pH 7,转速180 r/min,温度30.0℃。单菌株单基质降解试验表明,BW004菌可将200~1000 mg/L的喹啉在4-12小时降解98.8%以上。喹啉降解过程中,首先产生有机中间产物2-羟基喹啉和2,8-二羟基喹啉,同时杂原子氮转化为无机终产物氨氮。其后双环结构被破坏,溶液中的有机物在12~24小时被基本矿化。

A bacterial strain, BW004 that utilized quinoline as its sole carbon, nitrogen, and energy source, was isolated from the activated sludge of the coking wastewater treatment plant in Wuhan Iron and Steel Group, Hubei Province, China. The strain was identified as Pseudomonas sp. according to the 16S rRNA sequencing. The optimum degradation conditions determined by response surface methodology （RSM） were pH 7, rotation 180 r/rain, and temperature 30.0℃. The degradation experiments revealed that BW004 could degrade 200-1000 mg/L of quinoline over 98.8% within 4-12 hours. During the degradation, quinoline was first transformed to 2-hydroxyquinolin and 2,8-dihydroxyquinolin, and ammonium was released promptly as the final product of heterocyclic nitrogen; then the dual-ring structure was destroyed and all organics were mineralized in 12-24 hours.