黄泥混合海藻粉投喂刺参的稳定碳同位素研究

Stable Carbon Isotope Ratio Analysis of Yellow Soil as Feed Additive for Juvenile Sea Cucumber

大量海泥用作刺参(Apostichopus japonicus)饲料添加物对沿海底质环境造成一定的负面影响,寻找其替代物是当前刺参规模化育苗及养殖需要解决的问题之一。本研究用黄泥代替海泥混合孔石莼(Ulva pertusa)藻粉投喂刺参,比较两者对刺参的生长影响,采用稳定碳同位素比率法研究刺参对其吸收利用的情况。实验时,混合20%黄泥、海泥与80%海藻粉(干重)都按7.5%刺参幼参体重(湿重)投喂60d。结果,在黄泥组及海泥组中,刺参的特定生长率SGR分别为(1.95±0.13)%.d-1及(1.72±0.17)%.d-1;黄泥、孔石莼粉对刺参食物来源贡献分别为(9.78±1.37)%及(90.22±1.37)%,海泥、孔石莼粉对刺参食物来源贡献分别为(9.89±1.47)%及(90.11±1.47)%,以上各项指标在黄泥组刺参和海泥组间均无显著性差异(P>0.05)。结果表明,用黄泥代替海泥对刺参的生长影响及其被刺参吸收利用差异不显著,具备替换可行性。黄泥代替海泥能降低刺参饲料成本及加强对浅海底质环境的保护。

Stable carbon isotope ratio （B13C） was used to prove the feasibility of replacing sea mud with yellow soil in feed for juvenile sea cucumbers Apostichopus japonicus to eliminate negative effects of sea mud. A nurserypond experiment has been conducted at a mariculture site in Rongcheng City. The specific growth rate （SGR） of A. japonicus, feeding on 80% green algae powder （Ulva pertusa） plus 20% yellow soil or sea mud at 7.5% sea cucumber body weights for 60 d, was found to be （1.95±0.13）% d-1 and （1.72±0.17） % ·d-1, respectively. The contributions of yellow soil and sea mud to food sources of ju- veniles were （9.78±1.37） % and （9. 89±1.47）%, separately, while that of the green alga to food sources of juveniles were （90.22± 1.37）% and （90.11±1.47）%, respectively. There was no significant difference for the same index between treatments of sea mud and yellow soil （P〉0.05）. These results evidenced that replacing sea mud with yellow soil is feasible. Such a replacement can save feed cost and minimize the disturbance to seaboard.