鼻咽癌HPV感染及EGFR、VEGF、HIF-1a的表达研究

The study of HPV infection and the expression of EGFR and VEGF,HIF-1a in nasopharyngeal cancer

目的探讨HPV感染及表皮生长因子受体(EGFR)、血管内皮生长因子(VEGF)、乏氧诱导因子-1a(HIF-1a)的表达与鼻咽癌的关系。方法选择29例初治鼻咽癌组织标本(维吾尔族18例,汉族11例),采用原位杂交法检测HPV6/11、HPV16/18、HPV31/33感染情况,免疫组化法检测EGFR、VEGF、HIF-1a蛋白表达状态,分析EGFR、VEGF、HIF-1a蛋白表达与鼻咽癌临床特征的关系。结果全组鼻咽癌组织中HPV阳性检出率为0%,EGFR、VEGF、HIF-1a蛋白阳性率分别为44.8%、51.7%、48.3%,其中维、汉族鼻咽癌患者EGFR阳性表达率分别为27.8%、72.7%,差异具有统计学意义(P=0.027),维、汉族鼻咽癌患者VEGF、HIF-1a阳性表达率分别为66.7%、27.3%和50.0%、45.6%,差异无统计学意义(P=0.060、1.000)。T1+T2期患者EGFR、VEGF、HIF-1a阳性表达率分别为20.0%、20.0%、0.0%,T3+T4期患者阳性表达率分别为68.4%、68.4%、63.2%,差异均具有统计学意义(P<0.05),而与性别、年龄、N分期和临床分期无关。结论本地区鼻咽癌发生发展与HPV的感染的关联性有待进一步研究;EGFR、VEGF、HIF-1a在鼻咽癌中均有表达,维、汉族鼻咽癌EGFR阳性表达率有差异;EGFR、VEGF、HIF-1a表达与鼻咽癌的T分期可能有关。

Objective To explore the relationship between the infection of HPV, the expression of EGFR, VEGF, HIF-la and nasopharyngeal carcinoma development. Methods The infection of HPV6/11, 16/18, 31/33 were detected by in situ hybridization （ISH）, The expression status of EGFR, VEGF and HIF-la were examined by immunohistoehemistric method （IHC） in 29 patients with primary nasopharyngeal carci-noma tissue specimens （Uyghur 18, Han 11） ,and analyzed the relationship between EGFR, VEGF, HIF-la protein expression and nasopharyngeal clinical features. Results In all groups in nasopharyngeal carci-noma tissue the position expression of HPV were detected in 0%. The positive expression rate of EGFR, VEGF and HIF-la were 44.8% ,51.7% ,48.3%, of which the positive expression of EGFR in Uygur and Han were 27.8%, 72.7%, the difference were statistically significant （P=0. 027）, of VEGF, HIF-la in the Han and the positive expression rate respectively for 66.7%, 27.3%; 50%, 45.6%, the difference was not statistically significant （P =0. 060, 1. 000）. The positive expression rate of EGFR, VEGF, HIF- la of stage I and II patients were 20%, 20%, 0% and in that of stage III and IV patients were 68.4%, 68.4%, 63.2%, the difference were statistically significant （P d0.05）. Conclusion The development of nasopharyngeal carcinoma may be no significant correlation with the infection of HPV. EGFR, VEGF, HIF-la were expressed in nasopharyngeal carcinoma, and the positive expression of EGFR was statistically significant in Uygur and Han. The positive expression of EGFR, VEGF, HIF-la may be relevant with T stage.