不同提取工艺下白芷提取物的评价性质量分析

The Evaluative Quality Analysis of Angelicae Dahuricae Radix Extracts from Different Extraction Techniques

目的研究制定白芷提取物中欧前胡素含量测定方法,并对其不同提取工艺,进行评价性质量分析。方法以欧前胡素为指标,采用高效液相色谱法测定其含量;比较水提醇沉法、pH 8～9氨水提取醇沉法、pH 8～9氨水提取法、60%乙醇超声提取法和60%乙醇加热回流法等不同工艺的差异。结果 60%乙醇加热回流法欧前胡素含量较高,欧前胡素含量在2.5110～160.701μg范围里呈较好的线性关系;欧前胡素的回收率为98.07%。结论 60%乙醇加热回流法工艺所得白芷提取物中欧前胡素含量较高,欧前胡素转移率最高,提取方法合理、可行、效率高。

Objective To establish a method for content determination of the Imperatorin in the Angelicae Dahuricae Radix Extracts and make evaluative analysis among different extraction techniques. Methods Taking Imperatorin as a index, we use HPLC to determine its content. Then compare the difference among Water extraction - Alcohol precipitation,pH8-9 Ammonia water extraction - Alcohol precipitation, pH8-9 Ammonia water extraction, 60% Alcohol Ultrasound extraction and ,60% Alcohol Heating reflux method. Result The content of the Imperatorin from 60% Alcohol Heating reflux method is the higher. In the range of 2.5110-160.701μg,the Imperatorin shows a good linear relationship. The recovery of the Imperatorin is 98.07%. Conclusion The content of the Imperatorin from 60% Alcohol Heating reflux method ,is the higher and the transfer rate is the highest. The 60% Alcohol Heating reflux method is reasonable, feasible and efficient.