摘要
The optimal quantity of dietary lysine (Lys) and arginine (Arg), and the optimal ratio of dietary Lys to Arg for Pacific white shrimp Litopenaeus vannamei were investigated. Coated Lys and Arg were added to a basal diet (37.99% crude protein and 7.28% crude lipid) to provide graded levels of Lys and Arg. The experimental diets contained three Lys levels (2.51%, 2.11%, and 1.70% of total diet), and three Arg levels (1.41%, 1.80%, and 2.21% of total diet) and all combinations of these levels were tested. Pacific white shrimp, with a mean weight of 3.62±0.1 g, were randomly distributed in 36 fiberglass tanks with 30 shrimp per tank and reared on the experimental diets for 50 days. After the feeding trial, the growth performance, survival, feed conversion rate (FCR), body composition and protease and lipase activities in the hepatopancreases of the experimental shrimps were determined. The results show that weight gain (WG), specific growth rate (SGR), FCR, body protein, body Lys and Arg content were significantly affected by dietary Lys and Arg (P<0.05) and improved when dietary Lys and Arg levels were 2.11% 2.51% and 1.80% 2.21%, respectively. Protease and lipase activities in the hepatopancreases of the shrimps appeared higher when dietary Lys and Arg quantities were 2.11% 2.51% and 1.80% 2.21%, although the difference was not statistically significant (P>0.05). Therefore, according to our results, the optimal Lys and Arg quantities in the diet of Pacific white shrimp, L. vannamei, were considered to be 2.11%-2.51% and 1.80%- 2.21%, respectively, and the optimal ratio to be 1:0.88-1:1.05.
The optimal quantity of dietary lysine （Lys） and arginine （Arg）, and the optimal ratio of dietary Lys to Arg for Pacific white shrimp Litopenaeus vannamei were investigated. Coated Lys and Arg were added to a basal diet （37.99% crude protein and 7.28% crude lipid） to provide graded levels of Lys and Arg. The experimental diets contained three Lys levels （2.51%, 2.11%, and 1.70% of total diet）, and three Arg levels （1.41%, 1.80%, and 2.21% of total diet） and all combinations of these levels were tested. Pacific white shrimp, with a mean weight of 3.62±0.1 g, were randomly distributed in 36 fiberglass tanks with 30 shrimp per tank and reared on the experimental diets for 50 days. After the feeding trial, the growth performance, survival, feed conversion rate （FCR）, body composition and protease and lipase activities in the hepatopancreases of the experimental shrimps were determined. The results show that weight gain （WG）, specific growth rate （SGR）, FCR, body protein, body Lys and Arg content were significantly affected by dietary Lys and Arg （P〈0.05） and improved when dietary Lys and Arg levels were 2.11%-2.51% and 1.80%-2.21%, respectively. Protease and lipase activities in the hepatopancreases of the shrimps appeared higher when dietary Lys and Arg quantities were 2.11%-2.51% and 1.80%-2.21%, although the difference was not statistically significant （P〉0.05）. Therefore, according to our results, the optimal Lys and Arg quantities in the diet of Pacific white shrimp, L. vannamei, were considered to be 2.11%-2.51% and 1.80%- 2.21%, respectively, and the optimal ratio to be 1：0.88-1：1.05.
基金
Supported by the Research Foundation for Advanced Talents, Qingdao Agricultural University (No.631109)
