PTEN和C-erbB-2蛋白在子宫内膜癌的表达及临床意义

The expression and clinical significance of PTEN and C-erbB-2 protein in endometrial carcinoma

目的探讨PTEN和C-erbB-2蛋白在子宫内膜癌中表达的临床意义。方法采用免疫组化S-P法,分别在20例正常子宫内膜组织、20例子宫内膜不典型增生过长,50例术后子宫内膜癌组织标本中检测PTEN和C-erbB-2蛋白的表达情况。结果 (1)子宫内膜癌组织与子宫内膜不典型增生过长、正常子宫内膜组织比较,PTEN蛋白阳性率明显降低,有显著性差异。C-erbB-2蛋白阳性率明显增加,有显著性差异。(2)PTEN蛋白的表达与组织学分级、临床分级及临床分期都有关,而且随着组织学分级和临床分期的升高,PTEN的阳性表达出现显著降低,和有无淋巴结转移无关。(3)C-erbB-2蛋白的表达与临床分期有关,随着临床分期的增加,C-erbB-2蛋白的阳性表达显著升高,与组织学分级及淋巴结转移无显著相关。(4)在子宫内膜癌中PTEN与C-erbB-2蛋白的表达呈负相关。结论 PTEN蛋白失表达和C-erbB-2蛋白过表达在子宫内膜癌的发生、发展中发挥着一定的作用,两者的联合检测可能有助于子宫内膜癌的早期诊断,为预后评估提供帮助。

Objective：To study the expression and clinic significance of PTEN and C-erbB-2 protein in endometrial carcinoma and its relationships.Methods：Using immunohistochemistry test（s-p methods）,We examined the expression of PTEN and C-erbB-2 protein in 50 cases of endometrial carcinoma,20 cases of endometrial precancerous lesions and 20 cases of normal endometrium.Results：（1）In endometrial carcinoma tissues,the positive rates of PTEN protein expression was significantly lower than that of uterus atypical hyperplasial endometrium and normal endometrium tissues,but the positive rates of C-erbB-2 protein expression was significantly higher than that of uterus atypical hyperplasial endometrium and normal endometrium tissues.（2）The expression of PTEN protein correlated with histological grade and clinical stage,but not with lymph node metastasis.In endometrial carcinoma,with progression of clinical stage and increased histologic grade,the positive rate of PTEN protein expression was correspondingly decreased.（3）The overexpression of C-erbB-2 protein was correlated with clinical stage.In endometrial carcinoma,with progression of clinical stage,the positive rate of C-erbB-2 protein expression was correspondingly decreased,but there was no difference in statistics neither lymph node metastasis nor histological grade correlated with the expression of C-erbB-2 protein.（4） The expression of PTEN protein was negatively correlated with C-erbB-2 protein in endometrial carcinoma.Conclusion：The results suggest that absent PTEN and C-erbB-2 protein overexpression may play an important role in the genesis and development of endometrial carcinoma.There may be a synergetic action among these genes.Binded detections of PTEN and C-erbB-2 protein have positive effect on early diagnosis,prognosis assessment of endometrial carcinoma.