羊水预处理在羊水细胞培养瓶法中应用效果研究

Application effect research with amniotic fluid pretreatment in amniotic fluid cell culture bottle

目的研究羊水预处理在羊水细胞培养瓶法中的应用效果。方法采用羊水预处理培养瓶法(实验组)和未经处理羊水细胞培养瓶法(对照组)对100例18-24周的孕中期孕妇和152例25-32周的孕晚期孕妇进行羊水细胞培养。结果 100例孕18-24周羊水细胞培养实验组培养成功率高于对照组(P<0.05),实验组羊水细胞培养时间比对照组短(P<0.001),实验组有效核分裂数比对照组多(P<0.001);152例孕25-32周羊水细胞培养实验组培养成功率高于对照组(P<0.01),实验组羊水细胞培养时间比对照组短(P<0.001),实验组有效核分裂数比对照组多(P<0.001)。结论羊水预处理羊水细胞培养瓶法羊水细胞培养成功率高,细胞培养时间短,有效核分裂数多,可进行羊水产前诊断孕妇孕周范围增宽。

Objective：Research with amniotic fluid pretreatment in amniotic fluid cell culture bottle of application effect.Methods：100 samples of amniotic fluid from pregnant women during 18-24th-week gestation and 152 samples of amniotic fluid from pregnant women during 25-32th-week gestation were cultured in fluid pretreatment amniotic fluid cell culture bottle and untreated amniotic fluid cell culture bottle.Results：In 100 samples of pregnant women during 18-24th-week gestation.Culture success rate of the experimental group than the control group（P0.05）.Amniotic fluid cell culture time of the experimental group than the control group short（P0.001）.Effective mitotic count of the experimental group than the control group（P0.001）.In 152 samples of pregnant women during 25-32th-week gestation,Culture success rate of the experimental group than the control group（P0.01）,Amniotic fluid cell culture time of the experimental group than the control group short（P0.001）.Effective mitotic count of the experimental group than the control group（P0.001）.Conclusion：Amniotic fluid pretreatment in amniotic fluid cell culture bottle have high success rate,cell culture short time,the effective mitotic count more,and amniotic fluid prenatal diagnosis of pregnant women gestational weeksrange widened.