摘要
目的:研究大叶秦艽醇提取物对胶原诱导性关节炎大鼠的防治作用,并初步探讨其作用机制。方法:随机将96只大鼠分为正常组、模型组、白芍组及秦艽组。除正常组外,余各组用胶原乳液多点皮内注射建立关节炎模型。模型建立后第9天开始秦艽组以秦艽乙醇提物1.9ml·kg-1·d-1灌胃,白芍组给予白芍总苷胶囊内容物混悬液2ml·kg-1·d-1灌胃,模型组给予蒸馏水0.5ml·kg-1·d-1灌胃。给药后3周观察各组大鼠一般情况及血清IL-4及INF-γ水平。结果:模型组大鼠的踝关节及足趾关节明显肿胀、变形,秦艽组及白芍组与模型组比较关节肿胀程度明显减轻,INF-γ水平逐渐降低,IL-4水平明显升高,有显著统计学意义。结论:大叶秦艽醇提物能够在早期抑制胶原诱导性关节炎大鼠Th1型细胞因子INF-γ,促进Th2型细胞因子IL-4,减轻关节肿胀程度,改善滑膜炎症状,保护关节,体现治疗早期RA的优势。
Objective ： To research the effect of Large-leaved Gentian alcohol extract on collagen induced arthritis rats, and preliminary discussing the mechanism of action. Methods： 96 rats were randomly divided into the normal group, mode! group, the root of herbaceous peony group and Large-leaved Gentian group. In addition to the normal group, the more with collagen emulsion multipoint intradermal establish arthritis model. After the establishment of the model 9 day Large-leaved Gentian group to Large-leaved Gentian ethanol extract 1. 9ml ~ kg-1·d-1 lavage, root of herbaceous peony group was given root of herbaceous pe-ony total glycosides capsule contents2ml·kg-1·d-1 lavage, model group was given distilled water 0. 5ml - kg-1·d^-1 lavage. Immune after 14 days and 21 days, twenty eight days observation group rats in general and serum IL-4 and INF-γ level. Results： Immune after 14 days and 21 days, twenty eight days model rats, ankle joint swelling and toes markedly swollen, deformation, Large-leaved Gentian group and root of herbaceous peony group and model group is significantly reduce joint swelling, INF-γ level gradually reduce, IL-4 level increased significantly, a significant statistical significance. Conclusion： Large-leaved Gentian alcohol extract can early on inhibition of collagen induced arthritis rats Thl type cell factor IN-γ, promote the Th2 type cell factor IL-4, reduce joint swelling degree, improve the synovial inflammation shape, protect the joints, reflect the advantage of early treatment of RA.
出处
《四川中医》
2013年第6期72-74,共3页
Journal of Sichuan of Traditional Chinese Medicine
基金
甘肃省中医药管理局(编号:GZK-2010-3)
