醛糖还原酶1B10在肝癌组织中的表达与患者术前血清AFP水平的关系

Expression of AKR1B10 in hepatocellular carcinoma and its relationship with AFP level

目的探讨醛糖还原酶1B10(aldo-keto reductase family 1 member B10,AKR1B10)在肝癌组织中的表达及其与患者术前血清AFP水平的关系。方法采用免疫组化技术检测65例肝癌组织及其相应的癌旁肝组织、14例正常肝组织中AKR1B10蛋白的表达情况,分析AKR1B10蛋白在肝癌组织中的表达与患者术前血清AFP水平的关系。结果 AKR1B10蛋白在正常肝组织、癌旁肝组织及肝癌组织中的表达率和表达量均依次升高,两两比较差异均有统计学意义(P均<0.05)。在肝癌组织中AKR1B10蛋白的阳性表达率为76.9%,明显高于患者术前血清AFP的阳性率55.4%(P<0.05)。术前血清AFP阴性的29例患者中,有21例肝癌组织中AKR1B10蛋白阳性表达,阳性率为72.4%。AKR1B10蛋白在肝癌组织中的表达与患者术前血清AFP的水平无相关性(P>0.05)。结论 AKR1B10蛋白在正常肝组织、癌旁肝组织及肝癌组织中的表达依次升高,尤其是在肝癌患者术前血清AFP阴性的肝癌组织中表达较高,提示AKR1B10在肝癌的诊断方面具有潜在的临床应用价值。

Objective To explore the expression of aldo-keto reductase family 1 member B10 （AKR1BIO） in hepatocellular carci- noma （HCC）and its relationship with AFP level. Methods The expression of AKR1B10 protein was examined immunohistochemical- ly in 65 cases of human HCC and adjacent liver tissues,as well as in 14 cases of normal human liver tissue.Levels of AKR1B10 pro- tein expression in HCC and preoperative seIum AFP level were analyzed for a possible relationship. Results The expression rate and level of AKR1B10 protein were up-regulated in human HCC samples compared to the corresponding adjacent liver tissues,and in adjacent liver tissues compared to normal liver tissue（all P〈0.05）.Up-regulated AKR1BIO expression was found in 50 of 65 HCC cases （76.9%）, higher than the proportion of 55.4% of patients with detectable preoperative serum levels of AFP.Moreover,AKR1B10 protein was de- tected in 21 of 29 HCC cases （72.4%） that were negative for preoperative serum AFP.Statistical analysis showed that AKR1B10 pro- tein expression in HCC tissue was not correlated with preoperative serum AFP level. Conclusions AKR1B10 was up-regulated in the order：normal liver tissue〈tissue adjacent to HCC〈HCC tissue.The protein is often expressed in HCC,especially in patients who are negative for serum AFP.These results suggest that AKR1B10 may be useful for early diagnosis of HCC.