IGFBP4和PAPPA在非小细胞肺癌中的表达及临床意义

Expression and clinical significance of IGFBP4 and PAPPA in non-small cell lung cancer

目的研究IGFBP4和PAPPA基因在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中的表达水平,并分析其临床意义。方法提取55例手术治疗的NSCLC癌组织及癌旁组织的RNA,采用实时荧光定量PCR方法检测组织中IGFBP4和PAPPA的表达水平。结果 IGFBP4基因在NSCLC癌组织中的表达低于癌旁组织(P<0.05),但高于正常肺组织(P<0.05),在鳞癌组织中的表达明显高于腺癌组织,其表达水平在年龄、性别、临床分期、有无淋巴结转移中无明显差别(P>0.05),IGFBP4高表达组患者的生存期明显高于低表达组(P<0.05)。PAPPA基因在NSCLC癌组织中的表达高于癌旁组织和正常肺组织(P<0.05),其在癌旁组织和正常肺组织中的表达水平无差异(P>0.05),在腺癌组织中的表达明显高于腺鳞癌组织,在有淋巴结转移组的表达明显高于无淋巴结转移组(P<0.05),其表达水平在年龄、性别、临床分期中无明显差别,PAPPA高表达组患者生存期明显低于低表达组(P<0.05)。IGFBP4与PAPPA在NSCLC组织中的表达水平呈显著正相关(r=0.512,P=0.000)。结论 IGFBP4和PAPPA基因的表达与NSCLC的生长有关,可以作为判断NSCLC预后的参考指标。

Objective To investigate the expression and clinical significance of IGFBP4 and PAPPA in patients with non-small cell lung cancer（NSCLC）. Methods Total RNA was extracted from 55 pairs of NSCLC tissues and adjacent noncancerous tissues.IGFBP4 and PAPPA expression was detected using real-time quantitative RT-PCR. Results IGFBP4 expression was significantly lower in NSCLC tissue than in adjacent lung tissue,but significantly higher than in normal lung tissue（P〈0.O5）.IGFBP4 expression was signif- icantly higher in squamous carcinoma tissues than in adenocarcinoma.Furthermore, survival was longer in patients with high IGFBP4 expression than in those with low expression（P〈0.05）.However,IGFBP4 expression did not correlate with age,gender,histological dif- ferentiation grade or lymph node metastasis.PAPPA expression was significantly higher in NSCLC tissue than in adjacent or normal lung tissue（P〈0.05）.PAPPA expression was higher in cases of adenocarcinoma or lymph node metastasis than in cases of squamous carcinoma or cases without lymph node metastasis （P〈0.05）.Survival was shorter in patients expressing high levels of PAPPA than in those expressing low levels（P〈0.05）.However,PAPPA expression was not related with age,sex or clinical stage.There was a signifi- cant positive correlation between IGFBP4 and PAPPA expression in NSCLC tissues （r=0.512,P=0.000）. Conclusions IGFBP4 and PAPPA may correlate with tumor growth in NSCLC ,and may be useful as molecular prognostic indicators of NSCLC.