乌梅丸对TNBS诱导的结肠炎大鼠脾脏组织Bcl-2表达的影响

Effect of Wumeiwan on the expression of Bcl-2 in the spleen tissue of rats with TNBS-induced colitis

目的观察乌梅丸对2,4,6-三硝基苯磺酸(TNBS)诱导的结肠炎大鼠脾脏组织Bcl-2蛋白和mRNA表达的影响,研究其治疗结肠炎的免疫机制。方法将56只SD大鼠随机分成空白对照组、结肠炎模型组、美沙拉嗪组、乌梅丸组各14只。除空白对照组外,其他三组均用TNBS灌肠。模型建成2 d后,空白对照组和结肠炎模型组均以蒸馏水3 mL灌胃,美沙拉嗪组用50 g/L美莎拉嗪混悬液、乌梅丸组用0.515 g/L乌梅丸液3 mL灌胃,连续给药15 d后其取脾脏组织,应用Real time-PCR方法检测Bcl-2 mRNA表达,Western-blot技术检测Bcl-2蛋白表达。结果与空白对照组相比,结肠炎模型组Bcl-2 mRNA和蛋白表达升高(P均<0.05);与结肠炎模型组相比,乌梅丸组、美沙拉嗪组Bcl-2 mRNA和蛋白表达下降(P均<0.05),而乌梅丸组和美沙拉嗪组比较无统计学差异(P>0.05)。结论乌梅丸通过下调结肠炎大鼠脾脏组织Bcl-2 mRNA和蛋白表达,发挥治疗结肠炎的作用。

Objective To observe the effect of Wumeiwan on the expression of Bcl-2 protein and mRNA in the spleen tissue of rats with 2, 4, 6-trinitrobenzenesulfonic acid （TNBS）-induced colitis and to investigate its immune mechanism in treating colitis. Methods Fifty-six SD rats were randomly divided into the control group, colitis model group, mesalazine group and Wumeiwan group （ lg rats in each group）. Apart from the control group, rats in the other three groups were in- duced to experimental colitis by 2, 4, 6-trinitrobenzenesulfonic acid. And 2 days later, rats in the control group and colitis model group were administered intragastrically with normal saline at a dose of 3 mL, while rats in the mesalazine group and Wumeiwan group were intragastrically given mesalazine （50 g/L） and Wumeiwan （0. 515 g/L） at a dose of 3 mL, respec- tively. All rats were treated for 15 d. Spleen tissue samples were taken to detect the expression of mRNA of Bcl-2 by real- time PCR and the expression of Bcl-2 protein by Western blot. Results Compared with the control group, the expression of Bcl-2 mRNA and Bcl-2 protein was significantly increased in the colitis model group （ all P 〈 0.05 ） ; compared with the colitis model group, the expression of the mRNA and protein of Bcl-2 in the Wumeiwan and mesalazine groups was signifi- cantly decreased （ all P 〈 0.05）. And there were no significant differences in those between the Wumeiwan group and me- salazine group （P 〉 0. 05 ）. Conclusion Wumeiwan plays an important role in the treatment of rat TNBS-induced colitis by down-regulating the expression of the mRNA and protein of Bcl-2 in the spleen tissue.