幽门螺杆菌诱导大鼠胃上皮细胞凋亡与一氧化氮相关性研究

The correlation between gastric epithelial cell apoptosis induced by Helicobacter pylori in rats and nitric oxide

目的:研究幽门螺杆菌(Hp)感染诱导大鼠胃上皮细胞凋亡与一氧化氮(NO)的关系。方法:将60只Wistar大鼠随机分成正常对照组、Hp感染组和Hp根除组,每组20只。正常对照组正常喂养;Hp感染组大鼠按潘氏方法复制慢性胃炎模型,并参照陈氏方法感染Hp;Hp根除组大鼠感染Hp后采用四联疗法治疗。采用切口末端标记法(TUNEL)检测各组大鼠胃黏膜上皮细胞凋亡情况,硝酸还原酶法检测各组血清NO水平。结果:正常对照组、Hp根除组、Hp感染组的细胞凋亡指数和血清NO水平依次升高,且差异均有统计学意义(P<0.01);Hp感染组和Hp根除组胃上皮细胞凋亡指数与NO水平呈正相关关系(P<0.05)。结论:Hp感染可促进胃上皮细胞凋亡,促进NO生成可能是Hp促进细胞凋亡的机制之一。

Objective：To explore the correlation between gastric epithelial cell apoptosis induced by Helicobacter pylori （Hp） and nitric oxide （ NO ） in rats. Methods： Sixty wistar rats were randomly divided into the control group, Hp infection group and Hp eradication group（20 rats each group）. The rats in the control group were fed as normal. The chronic gastritis model rats in HP infection group were established then the rats were infected with Hp according to chen＇s methods. The rats in Hp eradication group were treated with quadruple chemotherapy after Hp infection. The gastric epithelial apoptosis of rats in all groups were determined by nick terminal lable technique（TUNEL） , the NO levels in serum of 3 groups were determined by nitrate reductase method. Results： The apoptotic index in gastric endothelial cell and levels of NO in serum in Hp infection group were significantly higher than those in control group （ P 〈 0.01 ）. Compared with the Hp infection group, the apoptotic index in gastric endothelial cell and levels of NO in serum in Hp eradication group were significantly reduced（ P 〈 0.01 ）. There were positively correlations between the apoptotic index and NO levels in Hp infection group and Hp eradication group （ P 〈 0.05 ）. Conclusions： Hp can induce the apoptosis of gastric endothelial cell. Promoting the NO generation may be one of inducing cell apoptosis mechanisms.