壳聚糖复合絮凝剂选择性絮凝双黄连水提取液的工艺研究

Selective flocculation processing technology of chitosan complex flocculant on Shuanghuanglian aqueous extract

目的优化壳聚糖复合絮凝剂用于双黄连水提取液选择性絮凝的最佳工艺条件。方法以绿原酸和黄芩苷保留率、鞣质脱除率为考察指标,分别考察复合絮凝剂制备工艺、絮凝剂用量、絮凝时间以及壳聚糖性能指标对絮凝效果的影响,优化选择性絮凝工艺条件。结果双黄连水提取液絮凝的优化工艺条件:膨润土经450℃灼烧4 h改性后负载壳聚糖制备复合絮凝剂,絮凝剂用量60 g/L,絮凝时间24 h,所用壳聚糖最佳性能指标:脱乙酰度95%、黏度60 cps。结论经高温改性的膨润土负载壳聚糖所制备的复合絮凝剂具有良好的选择性絮凝性能,絮凝效果显著优于《中国药典》2010年版方法。

Objective To optimize the selective flocculation processing technology of chitosan complex flocculant on Shuanghuanglian aqueous extract. Methods The impacts of preparation technology, the dosage of complex flocculant, the time of flocculation, and the performance index of chitosan on the flocculating effect of Shuanghuanglian aqueous extract were examined by using the retention rates of chlorogenetic acid and baicalin, as well as the elimination rate of tannin as observation indicators. Results The selective flocculation processing technology on Shnanghuanglian aqueous extract was optimized as follows： the bentonite was loaded by chitosan after it was burned for 4 h at 450 ℃, the dosage of complex flocculant was 60 g/L, the flocculating time was 24 h, and the best performance index ofchitosan is 95% in deacetylation, and 60 cps in viscosity. Conclusion The complex flocculant made of high temperature modified bentonite loaded by chitosan shows the good selectivity in flocculation capacity. The flocculating effect of this method on Shuanghnanglian aqueous extract is remarkably better than that in the one documented in Chinese Pharmacopoeia.