摘要
目的筛选油橄榄叶抗糖尿病活性部位。方法应用回流提取法和大孔吸附树脂技术,从油橄榄叶中提取6个部位(A～F)。对6个部位进行α-淀粉酶抑制实验、蛋白质非酶糖基化抑制实验和四氧嘧啶致小鼠糖尿病模型实验,综合评价和筛选抗糖尿病活性部位。结果 C～F部位对α-淀粉酶、蛋白质非酶糖基化均显示明显的抑制活性,其中以D部位活性最强,且呈量效关系。C、D部位降血糖作用较明显,且以D部位活性最显著。结论油橄榄叶具有潜在抗糖尿病活性,其中D部位的活性尤为显著。
Objective To screen the anti-diabetic effective fraction in the leaves of Olea europaea. Methods Different fractions （Frs. A-F） were prepared using reflux extraction and separation with macroporous resins, α-Amylase system, non-enzymatic glycation system, and alloxan-induced diabetic models of mice were used to evaluate the anti-diabetic activities of the different fractions, respectively. Results Frs. C-F fractions showed the significant inhibitory activities against a-amylase system and non-enzymatic glycation system. Especially, fraction D showed the best anti-diabetic activity compared with the other fractions, which also displayed a good dose-effect relationship. The anti-diabetic activities of Frs. C and D were both obvious and Fr. D showed more significance. Conclusion The leaves of O. europaea show the potential anti-diabetic activity and the most effective fraction is ascribed to Fr. D.
出处
《中草药》
CAS
CSCD
北大核心
2013年第13期1807-1810,共4页
Chinese Traditional and Herbal Drugs
基金
中国科学院"百人计划"资助项目(2007-0629141051)
国家自然科学基金资助项目(20974116)
关键词
油橄榄叶
抗糖尿病活性
Α-淀粉酶
蛋白质非酶糖基化
四氧嘧啶
糖尿病模型
leaves of Olea europaea
anti-diabetic activity
a-amylase
non-enzymatic glycosylation of protein
alloxan
diabetic model
