磷酸酯化银耳多糖对辐射损伤小鼠造血功能的保护作用

Protection of phosphate esterification polysaccharides from Tremella fuciformis on hematopoietic function in radiation-injured mice

目的研究磷酸酯化银耳多糖对辐射损伤小鼠造血功能的保护作用。方法小鼠随机分为6组:对照组,模型组,银耳多糖(10 mg/kg)阳性对照组,磷酸酯化银耳多糖低、中、高剂量(10、30、60 mg/kg)组。以137Csγ射线辐射前,给药组小鼠连续ip给药3 d,每天1次,辐射后第7天处死小鼠。以内源性脾结节形成、骨髓有核细胞数、白细胞数、脾脏指数及胸腺指数等为观察指标,探讨磷酸酯化银耳多糖对辐射小鼠造血功能的影响。结果与模型组相比,磷酸酯化银耳多糖各剂量组小鼠的骨髓有核细胞数、白细胞数、脾指数和胸腺指数均有不同程度的升高。结论磷酸酯化银耳多糖对辐射损伤小鼠造血功能具有一定的保护作用。

Objective To study the effect of phosphate esterification polysaccharides from Tremella fuciformis （PEPTF） on hematopoietic function in radiation-injured mice. Methods Rats were randomly divided into six groups： control, model, polysaccharides from T fuciformis （10 mg/kg） positive control, low-, mid-, and high-PEPTF （10, 30, and 60 mg/kg） groups. Before irradiated with ^137Cs γ-ray, rats were ip administered once daily for 3 d. On the day 7 after irradiation, the rats were sacrificed. Colony-forming unit of spleen, number of nucleated cells in bone marrow, number of white blood cells, spleen index, and thymus index were used as observation indexes to investigate the effect of PEPTF on hematopoietic function of mice. Results Compared with the model group, the number of the nucleated cells in bone marrow, the number of white blood cells, spleen index, and thymus index of mice increased markedly. Conclusion PEPTF have the protective effect on the hematopoietic function of radiation-injured mice.