黑果小檗总黄酮含量测定及体外抗氧化活性研究

Determination of total flavonoids and study on the antioxidation abiltity of berberis heteropoda schrenk in vitro

目的测定黑果小檗各部位中总黄酮含量并评价其体外抗氧化活性,为有效利用黑果小檗资源提供参考。方法采用紫外分光光度法测定黑果小檗中总黄酮的含量,并通过DPPH、羟自由基和氧自由基清除法评价黑果小檗提取物的抗氧化活性。结果黑果小檗果实中总黄酮含量较高;其次为茎皮及根皮;果实提取液的抗氧化活性较强,茎皮次之。结论黑果小檗提取物具有一定的抗氧化能力,本研究可为天然抗氧化剂的开发提供依据。

Objective To determine the content of total flavonoids in different parts of Berberis heteropoda Schrenk and to study the antioxidant activity so as to provide references for efficient utilization of the herbal plant. Methods We determined the total flavonoids of Berberis heteropoda Sehrenk by UV-Vis spectrophotometry, evaluated the antioxidant activity of the extracts from different parts of Ber- beris heteropoda Schrenk in scavenging DPPH radicals, hydroxy radicals and oxygen radicals. Results The contents of total flavonoids were richer in fruits of Berberis heteropoda Sehrenk than in stem bark and root bark. In addition, the extracts from the fruits of Berberis heteropoda Schrenk showed higher antioxidant activity than the stems. Conclusion The extracts of Berberis Heteropoda Schrenk show an- tioxidant capacity,thus can provide a basis for the development of natural antioxidants.