结缔组织生长因子表达水平与垂体腺瘤侵袭性之间关系

Difference of expression level of connective tissue growth factor between pituitary adenoma and aggressive pituitary adenoma

目的探讨结缔组织生长因子(CTGF)在垂体腺瘤中表达水平与垂体腺瘤侵袭性之间的关系,为侵袭性垂体腺瘤的诊断和生物学治疗提供理论依据。方法采用实时定量聚合酶链反应(Real-timePCR)和蛋白免疫印记杂交(Westernblot)方法检测16例非侵袭垂体腺瘤及52例侵袭性垂体腺瘤组织中CTGF的表达水平。结果 52例侵袭性垂体腺瘤和16例非侵袭性垂体腺瘤均可见CTGF的表达。在mRNA和蛋白水平上,侵袭性垂体腺瘤较非侵袭性垂体腺瘤CTGF表达高,且CTGF的表达差异有统计学意义(P<0.01)。结论 CTGF在侵袭性垂体腺瘤中表达明显高于非侵袭性垂体腺瘤,且与肿瘤的大小相关。

Objective To investigate the relationship between the expression of connective tissue growth factor（CTGF） and aggressive of human pituitary adenom, that provides the theoretical basis for the biological treatment of pituitary adenoma. Methods Quantitative real time PCR （ qRT- PCR） and Western blot were used to detecte the expression of CTGF in 16 cases of non-invasive pituitary adenoma and 52 cases of invasive pituitary adenoma（IPA）. Results CTGF expression was found in all cases of invasive pituitary adenomas and noninvasive pituitary adenomas. The expression of CTGF in invasive pituitary adenoma was higher than that of in noninvasive pituitary adenoma（P = 0.01 ）. Conclusions The expression of CTGF in invasive pituitary adenoma is significantly higher than that of in the noninvasive pituitary adenoma and related with the size of pituitary adenoma.