油樟叶挥发油β-环糊精包合物的制备工艺研究及稳定性考察

Study on preparation process and stability of β-cyclodextrin inclusion compound in volatile oil of Cinnamomum longepaniculatum leaves

研究油樟叶挥发油β-环糊精包合物的最佳制备工艺并考察其稳定性。采用饱和水溶法制备包合物,进行正交试验,以包合产率和包合率为筛选指标,优选包合工艺。使用红外分光光度法和薄层色谱法对包合效果进行评价,并考察包合物在强光、高温、高湿环境下的稳定性。最佳包合条件为1 mL油樟叶挥发油与8 gβ-环糊精进行包合,包合时间3 h,包合物温度40℃,β-环糊精和水的体积比1∶15。经红外分光光度法和薄层色谱法鉴定,形成了油樟叶挥发油β-环糊精包合物,并具有一定的抗光照性、热稳定性、湿稳定性。试验得出的最佳包合工艺操作简便,包合物稳定。

To study the optimum preparation process and stability of β-cyclodextrin inclusion compound in volatile oil of Cinna- momum longepaniculatum leaves. The saturated aqueous solution method was adopted to prepare inclusion compounds for an orthogonal test. The inclusion compound productivity and the inclusion rate were taken as indexes for screening the inclusion processes. The inclu- sion effect was evaluated with the infrared spectrophotometry and TLC, and the stability under conditions of high temperature, high hu- midity and strong light was detected. Under optimum preparation conditions for inclusion, the ratio between volatile oil andβ-cyclodex- trin was 1 ： 8 （ mL： g）, that between β-cyclodextrin and water was 1 ： 15, the inclusion temperature was 40 ℃, and the inclusion time was 3 h. The results of spectrophotometry and TLC showed that the optimum conditions can generateβ-cyclodextrin inclusion compound in volatile oil of C. longepaniculatum leaves with certain light resistance, thermo-stability and hygro-stability. Therefore the optimum inclusion process features simple operation and stable inclusion compounds.