HPLC同时测定正天丸中芍药苷、阿魏酸、升麻素苷、5-O-甲基维斯阿米醇苷的含量

Simultaneous determination of paeoniflorin,ferulic acid, prim-O-glucosylcimifugin and 4′-O-beta-glucopyranosyl-5-Omethylvisamminol in Zhengtian pills by HPLC

该研究以高效液相色谱同时测定正天丸(钩藤、白芍、川芎、当归、防风等)中芍药苷、阿魏酸、升麻素苷、5-O-甲基维斯阿米醇苷的含量。试验中采用Insertil ODS-C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.05%磷酸溶液为流动相,梯度洗脱,流速1.0 mL.min-1,柱温30℃,检测波长230 nm。由试验结果可知,芍药苷、阿魏酸、升麻素苷、5-O-甲基维斯阿米醇苷在11.37～170.5,2.188～32.82,2.896～43.44,3.000～45.00 mg.L-1峰面积与进样量线性关系良好,r分别为0.999 9,0.999 9,1.000 0,1.000 0;回收率(n=6)分别为102.3%(RSD 1.2%),99.71%(RSD 1.9%),101.2%(RSD 1.2%),99.40%(RSD 2.4%)。按所建方法对5批样品中上述4种成分的含量进行测定,结果均令人满意。该方法简便、准确,重复性好,可用于正天丸中芍药苷、阿魏酸、升麻素苷、5-O-甲基维斯阿米醇苷的质量控制。

To simultaneously determine paeoniflorin, ferulic acid, prim-O-glucosylcimifugin and 4＇-O-beta-glucopyranosyl-5- O-methylvisamminol in Zhengtian pills. In the test, Insertil ODS-C18column （4. 6 mm ×250 mm, 5μm） was adopted, with aeetoni- trile-0. 05% phosphoric acid solution as the mobile phase for gradient elution. The flow rate was 1.0 mL . min-1, the column tempera- ture was 30 ℃ and the detection wavelength was 230 nm. According to the results of the test, paeoniflorin, ferulic acid, prim-O-glu- cosyleimifugin and 4＇-O-beta-glucopyranosyl-5-O-methylvisamminol showed good linear relations between peak areas and sample sizes in 11.37-170. 5, 2. 188-32. 82, 2. 896-43.44, and 3.000-45. 00 mg . L-1 （ r =0. 999 9, 0. 999 9, 1. 0013 0, 1. 000 0） ,respectively. The average recoveries （n =6） were 102. 3% （RSD 1.2% ） ,99. 71% （RSD 1.9% ）, 101.2% （RSD 1.2% ）, and 99. 40% （RSD 2. 4% ）, respectively. The above four components were determined in five batches of samples by using the established method, with satisfactory results. The method was so simple, accurate and highly reproducible that it could be used for quality control of the four components in Zhengtian pills.