淫羊藿苷元磷脂复合物防治去卵巢骨质疏松大鼠骨丢失和提高骨质量的实验研究

Experimental study on effect of anhydroicaritin phytosomes in preventing and treating bone loss and enhancing bone quality in ovariectomized osteoporosis rats

目的:探讨淫羊藿苷元磷脂复合物防治去卵巢骨质疏松大鼠骨丢失和提高骨质量的作用。方法:72只雌性大鼠,随机分为6组:假手术组、模型组、雌二醇组和淫羊藿苷元磷脂复合物低、中、高剂量组。假手术组仅行假手术,其余5组行卵巢切除术。术后1周分别灌胃给予17β-雌二醇(2 mg.kg-1)和淫羊藿苷元磷脂复合物低、中、高剂量(15,30,60 mg.kg-1),连续给药3个月。测定血钙、血磷和碱性磷酸酶(ALP),尿钙(u-Ca)、尿磷(u-P)、尿脱氧吡啶酚(D-Pyr)和肌酐(Cr)。之后处死动物,取出股骨、腰椎和胫骨,分别测定股骨密度,骨钙、骨磷,腰椎生物力学性能,胫骨组织形态计量学参数。结果:与假手术组相比,模型组血清ALP,u-Ca,D-Pyr/Cr显著增加,子宫萎缩,股骨密度、骨钙、骨磷、腰椎生物力学参数(弹性载荷、最大载荷、断裂载荷、刚度)、胫骨上段静态参数(骨组织总面积、骨小梁面积、骨小梁周长)、动态参数(标记周长百分数、骨形成率)均降低。与模型组比较,淫羊藿苷元磷脂复合物中、高剂量和雌二醇组可使血清ALP,u-Ca,D-Pyr/Cr排出量降低,股骨密度、骨钙、骨磷、腰椎生物力学参数(弹性载荷、最大载荷、断裂载荷、刚度)、胫骨上段静态参数(骨组织总面积、骨小梁面积、骨小梁周长)、动态参数(标记周长百分数、骨形成率)均增加。雌二醇组大鼠子宫增生,淫羊藿苷元磷脂复合物组大鼠子宫没有增生性变化。结论:淫羊藿苷元磷脂复合物能抑制由于去卵巢雌激素缺乏引发的骨转换增强,提高骨密度,增强腰椎抗压生物力学性能,且长期服用对子宫没有刺激作用。

Objective： To explore the effect of anhydroicaritin phytosomes （AIP） in preventing and treating bone loss and en- hancing bone quality in ovariectomized osteoporosis rats. Method： Seventy-two SD female rats were randomly divided into 6 groups： the sham group, the model group, the estrogen group and AIP groups （low, middle, high）. The sham group was only sham operated, and the remaining five groups were ovariectomized. One week after the ovariectomy, the rats were given 17 beta-estrogen and AIP （ 15, 30,60 mg. kg-1 ） for consecutively three months, during which period their serum calcium （s-Ca）, serum phosphorus（ s-P）, alkaline phosphate （ ALP）, urine calcium （u-Ca）, urine phosphorus（u-P）, urinary deoxypyridinoline （D-Pyr） and creatinine （Cr） were de- tected. Subsequently, rats were sacrificed, and their thighbone, second lumbar vertebrate and forth lumbar vertebrate were collected to detect bone mineral density （BMD）, bone calcium （b-Ca）and phosphorus （b-P）, biomechanical properties and bone histomorphomet- ric parameters. Result： Compared with the sham group, the model group showed a significant increase in serum ALP, u-Ca and D-Pyr /Cr, and reduction in BMD of femur, b-Ca and b-P, biomechanical properties （ elastic load, maximum load, break load, stiffness）, static parameters （total tissue area, trabecular area, trabecular perimeter） and dynamic parameters （% L. Pm, BFR/BV and BFR/ TV）, with metratrophia. Compared with the model group, ALP high and middle-dose groups and the estrogen group showed a decrease in serum ALP, u-Ca and D-Pyr/Cr, and growth in BMD of femur, b-Ca and b-P, biomechanical properties of the forth lumbar verte- brae （elastic load, maximum load, break load, stiffness）, static parameters （total tissue area, trabecular area, trabecular perimeter） and dynamic parameters （% L. Pro, BFR/BV and BFR/TV）. The beta-estrogen group showed endometrial hyperplasia, whereas AIP groups showed no hyperplastic change. Conclusion： AIP could inhibit enhanced bone turnover induced by ovariectomy, improve BMD the biomechanical properties of vertebrae, without any stimulation on uterus.