人离体牙深低温保存的实验研究

An Experimental Study on Cryopreservation of Human Teeth in Vitro

目的 探索一种较佳的离体牙延期再植的保存方法。方法 10 0颗正常上下第一双尖牙 ,随机分成 5组 ,即对照组 ;微机控制降温 ,液氮 (- 196℃ )保存 1周、2周的离体牙 ;普通冰箱自然降温 ,液氮保存 1周、2周的离体牙。行活力检测 ,结果行 t检验。结果 离体牙在深低温保存后仍可观察到一定数量的有活性的正常形态的牙周膜细胞。各实验组分别与对照组比较 ,实验组组间比较 ,差别都无统计学意义。结论 人类离体牙在经过不同方法冻存一段时间后 ,其根面一定量的牙周膜细胞仍可保持活性 。

Objective To search for a better preservation method of human teeth in vitro for delayed reimplantation.Methods 100 first bicuspids were allocated into following 5 groups randomly:(1) Control Group,fresh in vitro teeth;(2) Experimental Group 1,stored at -196℃ in liquid nitrogen for 1 week;(3) Experimental Group Ⅱ,same as Experimental Group Ⅰ except stored for 2 weeks;(4) Experimental Group Ⅲ,first stored in the refrigerator, then transferred into liquid nitrogen for 1 week;(5) Experimental Group Ⅸ,same as Experimental Group Ⅲ,except stored for 2 weeks.The vitality test was carried out by observing the existence of the number of living PDL cells.Results PDL cells could be kept vital in normal form in all above five groups.There was no significant difference between neither the test groups and the control group, nor between Experimental Groups.Conclusion The cryopreservation of human in vitro teeth can be used for delayed reimplantation.