RP-HPLC同时测定不同产地肉桂中5种成分的含量

Simultaneous Determination of Five Components in Cinnamomum cassia from Different Spots by RP-HPLC

建立反相高效液相色谱同时测定不同产地肉桂中香豆酸、香豆素、肉桂醇、肉桂醛和桂皮酸的含量。色谱柱为Kromasil C18柱(4.6mm×250mm,5μm),流动相为甲醇∶水∶磷酸(45∶55∶0.1,V/V/V),流速0.8mL.min-1,检测波长280nm,柱温30℃。结果表明,香豆酸、香豆素、肉桂醇、肉桂醛和桂皮酸线性范围及线性相关系数分别为0.04096—0.4096μg(r=0.9983)、0.05848—0.5848μg(r=0.9999)、0.1141—1.1410μg(r=0.9999)、0.2000—2.0000μg(r=0.9999)和0.03552—0.3552μg(r=0.9999);平均加标回收率分别为98.2%、97.6%、99.1%、97.0%、98.8%,RSD(n=6)分别为2.4%、2.3%、1.5%、1.8%和2.0%。该方法准确、简便,数据可靠,可用于肉桂样品中香豆酸、香豆素、肉桂醇、肉桂醛和桂皮酸的定量分析。

RP-HPLC method for the determination of coumaric acid,coumarin,cinnamic alcohol,cinnamaldehyde and cinnamic acid in Cinnamomumc cassia from different spots was established by ultrasonic wave aided method.The separation was achieved on a Kromasil C18 column（4.6mm×250mm,5μm） at 30℃.The mobile phase was methanol-water-phosphoric acid（45∶55∶0.1,V/V/V） at a flow rate of 0.8mL·min-1.The detection wavelength was set at 210nm.The relationships and linear correlation coefficient of coumaric acid,coumarin,cinnamic alcohol,cinnamaldehyde and cinnamic acid were 0.04096—0.4096μg（r=0.9983）,0.05848—0.5848μg（r=0.9999）,0.1141—1.1410μg（r=0.9999）,0.2000—2.0000μg（r=0.9999）,0.03552—0.3552μg（r=0.9999）,respectively.The average recoveries were 98.2%、97.6%、99.1%、97.0%、98.8%,and RSD（n=6）were 2.4%、2.3%、1.5%、1.8%、2.0%,respectively.The proposed method is accurate,simple and promises to be applicable for the determination of coumaric acid,coumarin,cinnamic alcohol,cinnamaldehyde and cinnamic acid in Cinnamomumc assia simultaneously.