纳米金比色法测定6-巯基嘌呤

Determination of 6-Mercaptopurine with Gold Nanoparticles-Based Colorimetric Method

在pH 7.0的HAc-NaAc缓冲液溶中,6-巯基嘌呤可以通过Au-S键自组装于金纳米粒子的表面。以Cu2+作为交联剂,Cu2+与6-巯基嘌呤间的配位作用导致金纳米粒子快速发生团聚,金纳米粒子溶液的颜色由红色变为蓝色,最大吸收波长从520nm红移至660nm。660nm和520nm处吸光度的比值(A660/A520)与6-巯基嘌呤的浓度在5.0×10-8—1.0×10-6moL/L范围内存在线性关系。该方法测定6-巯基嘌呤的检出限为1.4×10-8moL/L,相对标准偏差为2.9%(2.0×10-7mol/L 6-巯基嘌呤溶液,n=11)。该方法已用于人血清中的6-巯基嘌呤含量的测定。

A colorimetric method was developed for the determination of 6-mercaptopurine,based upon the distance-dependent optical properties of gold nanoparticles（AuNPs）.In the presence of Cu2＋,6-mercaptopurine could rapidly induce the aggregation of gold nanoparticles in HAc-NaAc buffer solution（pH 7.0）,resulting in a color change of AuNPs solution from red to blue.The absorption peak of AuNPs shifted from 520nm to 660nm.A linear relationship between the ratio of absorbance at 660nm to 520 nm（A660/A520） and 6-mercaptopurine concentration was achieved in the range of 5.0×10-8—1.0×10-6moL/L.The detection limit was 1.4×10-8mol/L 6-mercaptopurine,the relative standard deviation was 2.9% for 2.0×10-7mol/L 6-mercaptopurine solution（n=11）.