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ISAV实时荧光RT-PCR检测方法的建立 被引量：2

Real-time fluorescent RT-PCR method for detection of ISAV
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摘要 为建立ISAV(传染性鲑鱼贫血症病毒)实时荧光定量PCR检测方法,针对ISAV基因序列保守区域设计一对特异性引物和一条特异性的探针,建立实时荧光RT-PCR探针检测法,并进行特异性、敏感性、重复性检测。结果表明:所建立的ISAV实时荧光RE-PCR探针检测法,引物和探针特异性良好,组内组间重复性良好,检出ISAV最小拷贝数为13拷贝。 A real-time PCR method was developed for the specific, sensitive and rapid detection of ISAV（Infec- tious salmon anemia virus）. A pair of specific primers and a specific probe was designed according to the ISAV gene conserved sequence. The real-time fluorescence PCR probe for detecting ISAV was established. The results showed that this method had good specificity and repeatability, the detection of ISAV minimum copy number was 13. Therefore, the study established the ISAV specific, sensitive and rapid real-time fluorescence PCR probe for fishery products.
作者 杜雄伟 李叶 庞艳华
机构地区 大连民族学院 辽宁出入境检验检疫局
出处 《食品与发酵工业》 CAS CSCD 北大核心 2013年第6期205-207,共3页 Food and Fermentation Industries
基金 中央高校自主基金项目(DC12010304)
关键词 传染性鲑鱼贫血症病毒 实时荧光RT-PCR Infectious salmon anemia virus, fluorescent PCR
分类号 R450 [医药卫生—治疗学]
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参考文献12

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同被引文献29

  • 1田洁莉,杨成辉.几种鲑鳟鱼类养殖过程中的病害综合防治[J].黑龙江水产,2004,23(3):30-30. 被引量：1
  • 2徐淑菲,孔繁德,高隆英,刘荭,陈信忠,龚艳青.SYBR-GreenⅠ实时荧光PCR检测传染性鲑鱼贫血病[J].检验检疫科学,2007,17(5):27-31. 被引量：10
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  • 10Kono T, Savan R, Sakai M, et al. Detection of white spot syndrome virus in shrimp by loop-mediated isothermal amplification. J Virol Methods, 2004, 115(1): 59-65.

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食品与发酵工业
2013年 第6期

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