栀子ERF基因的克隆及其在果实成熟过程中的表达

Molecular Cloning and Expression Analysis of ERF Transcription Factor Gene in Gardenia jasminoides During Fruit Development

乙烯反应元件因子(ethylene-responsive element-binding factor,ERF)是一类植物DNA结合蛋白,是乙烯信号传导过程中的一类转录因子,与植物的生长发育和生理过程有关。从栀子(Gardenia jasminoidesEllis)果实的cDNA文库中筛选获得栀子乙烯反应元件因子GjERF的cDNA。该基因全长962 bp,5'端非翻译区长82 bp,3'端非翻译区长100 bp,预测ORF为780 bp,编码259个氨基酸,分子量为28.6 kD。序列分析表明GjERF含有59个氨基酸构成的AP2/ERF结构域及N端的MC(MCGGAII)模体,它属于ERF家族的第Ⅶ亚类。RT-PCR分析表明GjERF基因在栀子成熟叶片中的表达高于在果实中的表达,并且在果实中的表达与果实的成熟过程无关。

Ethylene-responsive element-binding factor （ ERF ） genes constitute one of the DNA-binding protein gene families in plants, and are transcription factors associated with ethylene signal transduction, developmental and physiological processes. In this paper, a new member of the AP2/ERF transcription factor family, GjERF, was isolated from Gardenia jasminoides fruit cDNA library. The GjERF cDNA is 962 bp, contains a predicated 780 bp open reading frame that encodes a protein of 259 amino acids with calculated molecular mass of 28.6 kD, and with a 82 bp non-coding region at 5＇ end, a 100 bp of non-coding region flank at 3＇ end. Sequence analysis showed that GjERF contained an AP2/ ERF domain of 59 amino acids and a N- terminal MC motif （ MCGGAII ） . It belonged to a group VII protein in the ERF subfamily. The RT-PCR analysis indicated that the transcription of GjERF gene accumulated primarily in mature leaves of G.jasminoides, and was not related with fruit developmental stages.