中国恒河猴肠道菌群特征揭示

Characterization of Intestinal Microbiota in Feces from Captive Healthy Rhesus Macaques

运用变性梯度凝胶电泳(DGGE)和实时荧光定量(q-PCR)技术分析6个年龄组恒河猴粪便样品中主要细菌情况,揭示肠道微生态特点。16S rRNA基因V3可变区PCR-DGGE指纹图谱显示条带丰富,割胶62条,克隆测序后与GenBank序列进行比对后构建进化树显示所有62个条带、43个OTUs属于4个门:厚壁菌门,放线菌门,拟杆菌门以及螺旋体门。PCR-DGGE图谱泳道相似性分析及主成分分析(PCoA)显示婴幼龄组和孕期组聚类明显。Q-PCR结果显示梭菌属细菌和双歧杆菌最为丰富,肠球菌属最少。梭菌属clustersⅪⅤ,肠球菌属和拟杆菌-普氏菌属组间差异明显(P<0.01)。作为显示肠道健康水平标准的双歧杆菌和肠杆菌比值(B/E值)与同龄恒河猴相比,在孕期恒河猴组有明显的降低(P<0.01)。婴幼龄组和孕期组特点相似属首次发现。

With the denaturing gradient gel electrophnresis （ DGGE ） and real-time quantitative PCR （ q-PCR ） , we analyzed the predominant bacteria in fecal samples of 24 individuals divided into 6 groups. DGGE profiles showed abundant bands for the 16S rRNA gene V3 region, and the composition of the gut mierobiota was apparently different in each group. Furthermore, a clustering between infant and pregnancy was shown by Principal Coordinate Analysis （ PCoA ） . Identified in the DGGE gels by sequencing the V3 regions were overwhelmingly affiliated with Firmicutes, Actinobacteria and Bacteroidetes. Q-PCR indicated that Clostridium and Bifidobacterium were the dominant species, and the least dominant species was Enterococcusfaecali. C.clusters X/ V , E.faecalis and Bacteroides-Prevotella group have the most significant diversity among individuals （ P〈0.01 ） The Bifidobacteria/Enterobacteriaceae （ B/E ） ratio, which indicates microbial colonization resistance （ CR ） in the gut appeared to be lower than the peers, subadults and adults, in pregnancy （ P〈0.01 ） . The study revealed the characterization of Rhesus Macaquesintestinal microbiota. The characteristics of infant gut microbiota are remarkably similar to those found of the pregnancy groups.