摘要
目的探讨小肠缺血预处理对兔心肌缺血再灌注损伤的影响。方法健康成年新西兰大白兔18只,体重2.0~2.5 kg,随机分为3组(n=6),Ⅰ组阻断冠状动脉左前降支(LAD)30 min并再灌注2 h;Ⅱ组先阻断肠系膜上动脉(SMA)15 min并再灌注15 min(MAO15),其后操作同Ⅰ组;Ⅲ组先给予3次小肠缺血5 min再灌注5 min(3MAO5),其后操作同Ⅰ组。记录开胸前(T0)、阻断SMA后即刻(T1)、阻断SMA 30 min后(T2)、阻断LAD前(T3)及心肌再灌注2 h后(T4)的HR、和BP;分别于以上各时点采集静脉血测定肿瘤坏死因子(TNF-α)及乳酸脱氢酶(LDH)、肌酸激酶(CK)、肌酸激酶同工酶(CK-MB);T4时心肌染色,电镜下观察超微结构并测定心肌缺血范围和心肌梗死范围。结果与Ⅰ组和Ⅲ组分别比较,Ⅱ组心肌梗死范围明显减少(P〈0.05),T4时乳酸脱氢酶、肌酸激酶、肌酸激酶同工酶浓度明显降低,T3、T4时肿瘤坏死因子(TNF-α)浓度明显降低(P〈0.05),电镜下超微结构损伤较轻,心肌缺血范围与Ⅰ组、Ⅲ组差异无显著性。结论单次小肠缺血15min预处理能减轻心肌缺血再灌注损伤,抑制TNF-α浓度的升高。
【Objective】To observe the effect of small intestine ischemic pretreatment on the damage induced by myocardial ischemic-reperfusion.【Methods】Adult Newzeland rabbits were grouped in 3 groups randomly(n =6).Ischemic-reperfusion groupⅠ;Ⅱ preconditioning with a 15-min small intestine occlusion(MAO15);Ⅲpreconditioning with three cycles of 5-min small intestine occlusion and 5-min reperfusion.【Results】Compared with groupⅠ and groupⅢ,the myocardial size of groupⅡ was obviously smaller(P 0.05),the level of LDH,CK and CK-MB is obviously lower than the other group at T4,the level of TNF-α was obviously lower than the other group at T3,T4,and the area of myocardium at ischemic risk had no significant statistical difference among all of the groups.【Conclusion】The MAO15 can reduce the infarct size and the injury of IR,and it can inhibit the TNF-α after I/R.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2013年第16期25-28,共4页
China Journal of Modern Medicine
关键词
再灌注损伤
缺血预处理
心肌
肿瘤坏死因子Α
reperfusion injury
ischemic precondition
myocardium
tumor necrosis factor-alpha