乳腺癌中bcl-w的mRNA和蛋白的表达及其临床意义

The mRNA and protein expression of bcl-w and its clinical value in breast cancer

目的探讨乳腺癌组织中bcl-w基因mRNA及蛋白表达情况及其临床意义。方法应用Re-al-time PCR及Western blotting等分子生物学技术检测抗凋亡基因bcl-w的转录及翻译水平(即mRNA及蛋白表达水平)。结果①mRNA表达情况:bcl-w的mRNA表达水平在各期乳腺癌组中均高于正常乳腺组(P<0.05);而乳腺癌TNM临床分期中,II期、III期和IV期均高于I期(P<0.05),而前三者之间差异无显著性(P>0.05);②蛋白表达情况:bcl-w的蛋白表达水平在各期乳腺癌组中均高于正常乳腺组(P<0.05);而乳腺癌TNM临床分期中,II期、III期和IV期均高于I期(P<0.05),而前三者之间差异无显著性(P>0.05)。结论bcl-w在乳腺癌发生及发展恶化过程发挥着重要作用,抑制bcl-w表达水平,可作为乳腺癌的一种潜在治疗方法。

【Objectives】To study the mRNA and protein expression of bcl-w and their clinical value in breast cancer.【Methods】The mRNA expression of bcl-w was detected with Real-time PCR,and the protein expression of bcl-w was tested with Western blotting.【Results】①mRNA expression： the bcl-w mRNA expression of the four stages of breast cancer were all higher than the normal breast（P 0.05）;and the stage II,III and IV were higher than stage I（P 0.05）,whereas there were no statistically significant difference among the stage II,III and IV;②protein expression： The bcl-w protein expression of the four stages of breast cancer were all higher than the normal breast（P 0.05）;and the stage II,III and IV were higher than stage I（P 0.05）,whereas there were no statistically significant difference among the stage II,III and IV.【Conclusions】Bcl-w may play an important role in the carcinogenesis and progression of breast cancer.And the inhibition of bcl-w could be used as a potential treatment of breast cancer.