中华鳖腐皮病的病原鉴定与药敏试验

Studies on Pathogenic Identification and Drug Sensitivity Analysis of Skin Fester Disease in Trionyx sinensis

为了确定中华鳖(Trionyx sinensis)腐皮病的主要致病菌,从湖南省湘阴县中华鳖养殖场采取已呈明显发病症状且频死的中华鳖10只,从背甲和腹甲病灶处分离到6株菌株,通过分离纯化培养,挑选出2株优势菌株,经形态特征、生理生化特性和人工感染试验,鉴定为嗜水气单胞菌(Aeromonas hydrophila)和产碱杆菌(Bacillus al-caligenes)。药敏试验结果表明,这2种致病菌均对青霉素、红霉素、卡那霉素、庆大霉素、丙氟哌酸等抗生素高度敏感,可作为治疗该病的推荐药物。

In order to identify the main pathogenic bacteria of skin fester disease in Trionyx sinensis, ten dying and symptoms Trionyx sinensis were selected from the farm in Xiangyin County, Hunan Province and six bacterial strains were isolated from their carapace and plastron lesions. By cultivating of separation and purification, two predominant strains were selected and identified by strain morphological characteristics, physiological and biochemical characteristics and artificial infection drug test, and Aeromonas hydrophila and Bacillus alcaligenes had been proved to be the pathogenic bacteria. The results of drug sensitivity tests showed that the two pathogens were highly sensitive to Penicillin, Erythromycin, Kanamycin, Gentamicin and Ciprofloracin, and these could be the chief drugs for the treatment of SFD （skin fester disease）.