转抗坏血酸过氧化物酶基因(APX)菊苣抗旱相关生理特性

Characterize the Drought Resistance of Ascorbate Peroxidase Gene (APX) Transgenic Cichorium intybus

在获得转抗坏血酸过氧化物酶基因(APX)菊苣株系的基础上,对3个转基因株系的抗旱相关生理特性进行分析。结果表明:正常供水条件下,转基因株系与非转基因植株各项生理指标差异不显著;干旱条件下,3个转基因株系菊苣叶片APX质量摩尔浓度均显著高于非转基因对照。3个转基因株系中有2个株系丙二醛(MDA)质量摩尔浓度低于非转基因对照,表明APX基因可显著提高菊苣APX活性,降低氧自由基浓度,减轻对细胞的伤害。3个转基因株系叶片相对含水量、脯氨酸质量分数、叶绿素质量分数、单株幼苗的根系生长量,均高于非转基因对照。可见,转APX基因菊苣具有较强的抗旱能力,从而验证APX基因的抗旱功能,为转基因材料应用于菊苣的抗旱育种提供依据。

On the basis of having the ascorbate peroxidase （APX） gene transgenic Cichorium intybus, physiological characteristics of drought resistance of three transgenic strains were analyzed. In normal water supply, the differences between transgenic and non-transgenic plants were not significant; in drought condition, APX molality in three transgenic Cichorium intybus leaves was significantly higher than non-transgenic control group, and this indicated that APX gene could increase the Cichorium in- tybus APX activity significantly, while decrease the oxygen radical concentration, and reduce the harmfulness to cells. Measurement results showed that proline mass fraction, leaf relative water content, chlorophyll mass fraction,and single seedling root system increment of three transgenic plants were all higher than that of non-transgenic control plants. Malondialdehyde content in two of three transgenic plants was lower than non-transgenic control plants. As a conclusion of above results, a- scorbate peroxidase gene transgenic Cichorium intybus had stronger drought resistance ability, and this validated the drought resistance function of APX gene, and gave a basis for the transgenic materials applied in the drought resistance breeding of Cichorium intybus.