不同人工饲料对花绒寄甲成虫肠道微生物群落组成的影响

Effect of Different Artificial Diets on Intestinal Microbial Community of Dastarcus helophoroides

研究不同饲料对花绒寄甲[Dastarcus helophoroides(Fairmaire)]成虫肠道微生物区系的影响。人工配制4种花绒寄甲成虫饲料(Ⅰ、Ⅱ、Ⅲ和Ⅳ),主要成分分别为蚕蛹粉、蟋蟀粉、替代寄主粉和蚂蚁粉,分别饲养花绒寄甲成虫。无菌取其肠道,进行肠道细菌16SrDNA全长PCR扩增和16SrDNA V3可变区的PCR扩增,扩增产物经16SrDNA作为分子标记的变性梯度凝胶电泳(DGGE)分离后再进行相似性分析。结果表明,从成虫肠道共得到11种细菌,分别属于德库菌属(Desemzia)、葡萄球菌属(Staphylococcus)、不粘柄菌属(Asticcacaulis)、纤维弧菌属(Cellvibrio)、动性杆菌属(Planomicrobium)、橙单胞菌属(Aurantimonas)和埃希氏菌属(Escherichia)。经相似性分析后,饲喂饲料Ⅰ(蚕蛹粉)和饲料Ⅱ(蟋蟀粉)的成虫肠道细菌区系相似性为64%;饲喂饲料Ⅲ(替代寄主粉)和饲料Ⅳ(蚂蚁粉)的成虫肠道细菌区系的相似性为61%。饲料Ⅰ、Ⅱ喂养的成虫和饲料Ⅲ、Ⅳ喂养的成虫肠道菌群差异性较大,相似性为21%。可见,不同饲料对花绒寄甲成虫肠道微生物群落组成有很大的影响。

The experiment was conducted to determine the effect of different artificial diets on intestinal bacterial communities in adults of Dastarcus helophoroides. Four diets （diets I , Ⅱ , Ⅲ and Ⅳ ） were prepared as the adult diet of Dastarcus helophoroides and the major components were Silkworm pupa-powder,Crickets powder,darkling beatle pupal-powder and ant powder. The intestinal bacterial communities were assayed by PCR-DGGE to determine whether different artificial diets could influence the bacterial communities. The results revealed that Desemzia, Staphylococcus, Asticcacaulis, Cellvibrio, Planomicrobium, Aurantimonas and Escherichia were isolated from the gut of adults. The similarity of the intestinal bacterial communities of the adults fed diet Ⅰ （Silkworm pupa powder） and diet Ⅱ （Cricket powder） , diet Ⅲ （darkling beetle pupal powder） and diet IV （ant powder） was 640% and 61%,respectively. Diet Ⅰ , Ⅱ and diet Ⅲ ,Ⅳ did not cluster,and their similarity was 21%. Specifically, the quantities intestinal bacterial were different in the adults fed on different diets.