野生软枣猕猴桃组织培养及褐变处理

Efficiency Plant Regeneration and Browning Condiction of Actinidia arguta

以野生软枣猕猴桃嫩芽、幼嫩叶片以及茎段作为外植体,研究野生软枣猕猴桃组织培养整个过程,以建立快速高效的野生软枣猕猴桃再生体系。结果表明:最适宜的诱导叶片、茎段愈伤组织的培养基分别为MS+0.5mg/L6-BA+1.0mg/LNAA和MS+0.5mg/L6-BA+0.1mg/LNAA;诱导出愈伤组织在MS+0.6mg/L6-BA+0.05mg/LNAA培养基上能很好地分化不定芽苗;诱导幼芽产生丛生芽的培养基为MS+1.0mg/L6-BA+0.1mg/LNAA;较适宜的生根培养基为1/2MS。愈伤组织继代中发现细胞生长素NAA可以防止愈伤褐化。在愈伤组织分化中,发现将分化出的芽苗再次愈伤化,可以提高分化率。

The experiment chose wild soft dates kiwi as a tender plant, and as a young leaves and stem section for explant, the wild soft tissue culture to the whole process dates kiwi, established a rapid and efficient wild soft dates kiwi regeneration system. The results showed that： the most suitable induction blade, stem section callus culture medium were respectively for MS＋0.5 mg/L 6-BA＋1.0 mg/L NAA and MS＋0.5 mg/L 6-BA＋ 0.1 mg/L NAA; induction of callus in MS ＋ 0.6 mg/L 6-BA ＋ 0.05 mg/L NAA medium could be a very good differentiation in vitro shoot seedlings; bud induction multiple shoot clumps of culture medium was produced for MS ＋ 1.0 mg/L 6-BA ＋ 0.1 mg/L NAA; the better suitable for 1/2 MS root medium. In later experiment of callus found that auxin NAA could prevent callus from browning. In the callus differentiation, it was found that differentiated bud seeding to callus again, could improve the differentiation rate.