绿色木霉Sn-9106固态发酵中药残渣产纤维素酶研究

Studies on cellulase production by an isolated strain of Trichoderma viride Sn-9106 in solid-state fermination of residue of Chinese herbs

对绿色木霉Sn-9106固态发酵中药残渣产纤维素酶的可行性进行了研究。以滤纸酶为纤维素酶活性指标,麸皮、蛋白胨、KH2PO4添加量为影响因子,先采取单因素实验确定3种影响因子的最佳浓度,然后通过相应面法(RSM)优化产酶最佳条件。结果表明,当最大酶活力为12.3IU/g时所需固体发酵基质中麸皮、蛋白胨及KH2PO4的浓度分别为19.80g/L、2.06g/L、2.90g/L,与优化前培养基相比,纤维素酶产量提高了近3倍。

The Trichoderma viride Sn-9106 with high cellulase actibity was employed to produce enzyme on residues of Chinese herbs as substrate in solid state fermentation.Residues of Chinese herbs and peptone were found to be the best combination of carbon and nitrogen source for the production of cellulase.The nutrient composition of medium was optimized using response surface methodology（RSM）.A fractional factorial design was applied to elucidate the nutrient medium components that significantly affect cellulase production.The composition of nutrient fermentation medium optimized by response surface methodology was as follow（g/L）： wheat bran 19.80,peptone 2.06 and KH2PO42.90.Compared to the original medium,the cellulase activity increased from 8.83 IU/ml to 12.60 IU/ml.