摘要
利用4-硝基苯基-β-D吡喃葡萄糖苷为底物测定酵母中的β-葡萄糖苷酶,研究8株酿酒酵母在上清液、壁膜间隙和细胞内的β-葡萄糖苷酶活性及氧气对酿酒酵母产β-葡萄糖苷酶的影响。结果表明β-葡萄糖苷主要位于细胞间隙和细胞内,酿酒酵母M4产β-葡萄糖苷酶最高,为4.1μmol pNP.mL-1.h-1。氧气显著促进酿酒酵母合成β-葡萄糖苷酶,且相对于厌氧条件,有氧条件下酿酒酵母M4的β-葡萄糖苷酶增加了4.51倍。
To study the enzyme location and the effect of oxygen on β-glucosidase activity,eight Saccharomyces cerevisiae stains were evaluated using 4-nitrophenyl-β-D glucopiranoside（pNPG） as substrate in supernatant,periplasmic space and intracellular under aerobic and anaerobic conditions.The results showed that most enzymes were located in both periplasmic space and intracellular.S.cerevisiae M4 had the highest β-glucosidase activitiy（4.1μmol pNP.mL-1.h-1）.Aerobic growth condition significantly stimulated β-glycosidase biosynthesis in S.cerevisiae stains.S.cerevisiae M4 showed 4.51-fold more enzymatic activity under aerobic condition than under anaerobic condition.
出处
《中国酿造》
CAS
2013年第6期28-30,共3页
China Brewing
基金
国家自然科学基金(31271917)
国家葡萄产业技术体系建设专项经费(CARS-30-jg-3)
关键词
酿酒酵母
Β-葡萄糖苷酶
酶的分布
氧气
Saccharomyces cerevisiae
β-glucosidase activity
enzyme activity location
oxygen
