陈醋生高粱糖化菌株的筛选

Screening of strains for orghum saccharifying in mature vinegar production

选择产生淀粉糖化酶较多的3种黑曲霉菌株H303-4-3、AS.3324和CICC2137,对3株菌株产酶条件进行优化使菌株的产糖化酶能力得到提高,经优化后H303-4-3菌株的糖化酶活力最高。在麸皮与豆粕的比例为4∶1,麸皮与水分的比例为15∶10,pH值5,装瓶量为15g的条件下,接种培养4d的菌株,30℃培养4d,H303-4-3的糖化酶活力达2783.63U/g。

Three kinds of Aspergillus niger strain with good starch glucoamylase producing ability H303-4-3,AS.3324 and CICC2137 were chosen for producing conditions optimization.And after that strain H303-4-3 was found with highest glucoamylase producing ability.The producing conditions were as follow： Bran and soybean meal ratio of 4∶1,a ratio of bran and water 15∶10,pH value of 5,bottling capacity of 15g.In those conditions,inoculated strains cultured 4d,then culture for 4d under 30℃,glucoamylase activity of strain H303-4-3 could reach 2783.63U/g.