人参茎叶总皂苷对负重游泳小鼠抗疲劳作用及其机制

THE ANTIFATIGUE EFFECTS AND MECHANISM OF GINSENG STEM AND LEAF SAPONIN ON WEIGHT-BEARING SWIMMING MICE

目的探讨人参茎叶总皂苷(GSLS)的抗疲劳作用及其机制。方法清洁级昆明种小鼠40只,雌雄各半,体质量(21±3)g,随机分为对照组(NC组)、GSLS 10mg/(kg.d)组(LD组)、GSLS 40mg/(kg.d)组(MD组)、GSLS 160mg/(kg.d)组(HD组),每组又分为7d和28d组。NC组给予生理盐水灌胃7d或28d,LD、MD、HD组给予相应剂量的GSLS连续灌胃7d或28d。测定各组小鼠力竭游泳时间、肌糖原、乳酸脱氢酶(LDH)、血尿素氮(BUN)等指标的变化。结果 GSLS灌胃28d后,MD、HD组小鼠体内肌糖原水平均有显著提高(F=6.62,q=6.565、5.102,P<0.01)。MD组运动后LDH活性明显提高(F=6.91,q=9.021,P<0.01),运动后血BUN的水平降低,加速体内BUN的清除(F=5.25,q=4.329,P<0.05)。结论 GSLS通过调节糖原储存、增加LDH活性、调整血尿素氮含量而产生抗疲劳作用,其中以40mg/(kg.d)为最佳用量,而160mg/(kg.d)灌胃28d可能存在一定毒性。

Objective To explore the antifatigue effects and the mechanism of Ginseng stem and leaf saponin （GSLS）. Methods Twenty male and 20 female Kunming clean mice with the body mass Of （21 ± 3）g were randomly divided into four groups： control group （NC group）, GSLS 10 mg/（kg · d） （LD group）, GSLS 40 mg/（kg · d） （MD group）, and GSLS 160 mg/ （kg · d） （HD group）. In each group, it was subdivided into 7-clay and 28-day subgroup. The mice in NC group were given intragas- trie administration of normal saline for seven or 28 days. Those in LD, MD and HD group were respectively given intragastric ad- ministration of corresponding dose of GSLS for seven or 28 days. The time of exhausting swimming, the levels of muscle glycogen, serum lactic dehydrogenase （LDH） and blood urea nitrogen （BUN） were measured. Results After 28-day GSLS administra- tion, the levels of muscle glycogen in the mice in MD and HD group markedly elevated （F=6.62;q=6.565,5.102;P〈0.01）, and postexercise serum LDH activity in MD raised （F =6.91 ;q = 9.021;P〈0.01）, BUN lowered, and BUN clearance rate accelerated （F=5.25;q=4.329;P〈0.05）. Conclusion GSLS plays an anti-fatigue action through regulating glycogen storage, increasing LDH activity, and adjusting BUN level. The optimized dosage of GSLS was 40 mg/（kg · d）. The dosage of 160 mg/（kg · d） for 28 days might cause a certain toxic reaction.