fos-GFP转基因小鼠学习记忆能力的变化

THE CHANGES OF LEARNING AND MEMORY ABILITY IN fos-GFP TRANSGENIC MICE

目的研究fos-GFP转基因小鼠学习记忆能力的变化。方法转基因组和对照组小鼠分别采用高架十字迷宫、旷场实验、水迷宫测试、条件性场景恐惧记忆测试方法进行小鼠的自发活动、焦虑状态及学习记忆能力测试。结果 fos-GFP转基因小鼠与正常小鼠旷场内运动距离、运动速度、在旷场边缘区逗留的时间及在十字迷宫中开放臂探究的时间比较差异无显著性(P>0.05);两组小鼠水迷宫训练中各时间点找到平台的潜伏期比较差异无显著性(P>0.05);两组小鼠水迷宫测试中在各象限时间百分比比较,差异无显著性(P>0.05)。fos-GFP转基因小鼠在无特定场景提前接触的条件恐惧测试中的僵立时间与正常小鼠比较差异有显著性(t=9.48,P<0.001)。结论 fos-GFP转基因小鼠海马依赖性的联合型学习记忆能力有所提高。

Objective To study the changes of the ability on learning and memory in fos GFP transgenic mice. Me- thods The mice in the fos-GFP group and the control were tested by using overhead cross maze, open field, and Morris Water Maze. The spontaneous activity, anxiety state, the ability of learning and memory were tested as well. Results The differences between fos-GFP mice and the controls were not significant in terms of distance of motion within the field, velocity,duration of stay at the marginal zone, and duration of open arm in the maze （P〉0.05）. The difference between the mice in the two groups was not significant with regard to latency of finding the flat at each time point （P〉0.05）, and the percentage of duration in target quadrant in Morris Water Maze （P 〉0.05）, and similar freezing time in contextual fear conditioning test （P〉0.05）. The fos-GFP mice showed higher freezing level than normal controls in no-exposed fear conditioning （t=9.48,P〈0.01）. Conclusion The ability of hippoeampus-dependent associative learning and memory in fos-GFP transgenic mice was somewhat improved.